考察抗坏血酸钠对裂殖壶菌发酵产DHA的影响任务书
2020-05-24 12:16:44
1. 毕业设计(论文)的内容和要求
内容:
1、添加不同浓度抗坏血酸钠的发酵差异。
2、考察添加抗坏血酸钠对发酵过程中总抗氧化能力(t-aoc)和氧自由基(ros)的影响。
2. 参考文献
[1] Huangfu, J., Liu, J., Peng, C., Suen, Y. L.,Wang, M., Jiang, Y., Chen, Z.-Y., amp; Chen, F. (2013). DHA-rich marine microalga Schizochytrium mangrovei possesses anti-ageing effects on Drosophila melanogaster. Journal of Functional Foods, 5, 888#8211;896. [2] Johnson, M. B., amp; Wen, Z. (2009). Production of biodiesel fuel from the microalga Schizochytrium limacinum by direct transesterification of algal biomass. Energy and Fuels, 23, 5179#8211;5183. [3] Kawashima, H., Akimoto, K., Shirasaka, N., amp; Shimizu, S. (1996). Inhibitory effects of alkyl gallate and its derivatives on fatty acid desaturation. Biochimica et Biophysica Acta (BBA) #8211; Lipidsand Lipid Metabolism, 1299, 34#8211;38. [4] Scott, S. D., Armenta, R. E., Berryman, K. T., amp; Norman, A. W. (2011). Use of raw glycerol to produce oil rich in polyunsaturated fatty acids by a thraustochytrid. Enzyme and Microbial Technology, 48, 267#8211;272. [5] (4) Chitranjali, T.; Anoop, P. A.; Kurup, G. M. Omega-3 fatty acid concentrate from Dunaliella salina possesses anti-inflammatory properties including blockade of NF-κB nuclear translocation. Immunopharmacol. Immunotoxicol. 2015, 37, 81#8722;89. [6] (6) Ganuza, E.; Anderson, A. J.; Ratledge, C. High-cell-density cultivation of Schizochytrium sp. in an ammonium/pH-auxostat fedbatch system. Biotechnol. Lett. 2008, 30, 1559#8722;1564. [7] Hillig, F.; Porscha, N.; Junne, S.; Neubauer, P. Growth and docosahexaenoic acid production performance of the heterotrophic marine microalgae Crypthecodinium cohnii in the wave-mixed single-use reactor CELL-tainer. Eng. Life Sci. 2014, 14, 254#8722;263. [8] da Silva, T. L.; Mendes, A.; Mendes, R. L.; Calado, V.; Alves, S. S.; Vasconcelos, J. M. T.; Reis, A. Effect of n-dodecane on Crypthecodinium cohnii fermentations and DHA production. J. Ind. Microbiol. Biotechnol. 2006, 33, 408#8722;416. [9] Huang, Q.; Gao, B.; Jie, Q.; Wei, B. Y.; Fan, J.; Zhang, H. Y.; Zhang, J. K.; Li, X. J.; Shi, J.; Luo, Z. J.; Yang, L.; Liu, J. Ginsenoside-Rb2 displays anti-osteoporosis effects through reducing oxidative damage and bone-resorbing cytokines during osteogenesis. Bone 2014, 66, 306#8722;314. [10] Wynn, J. P.; Kendrick, A.; Ratledge, C. Sesamol as an inhibitor of growth and lipid metabolism in Mucor circinelloides via its action on malic enzyme. Lipids 1997, 32, 605#8722;610. [11] L.-J. Ren, H. Huang, A.-H. Xiao, M. Lian, L.-J. Jin, X.-J. Ji, Enhanced docosahexaenoic acid production by reinforcing acetyl-CoA and NADPH supply in Schizochytrium sp. HX-308, Bioprocess Biosyst. Eng. 32 (6) (2009) 837#8211;843. [12] M. Chen, H. Tang, H. Ma, T.C. Holland, K.Y.S. Ng, S.O. Salley, Effect of nutrients on growth and lipid accumulation in the green algae Dunaliella tertiolecta, Bioresour. Technol. 102 (2) (2011) 1649#8211;1655. [13] E. Ryckebosch, K. Muylaert, I. Foubert, Optimization of an analytical procedure for extraction of lipids from microalgae, J. Am. Oil Chem. Soc. 89 (2) (2011) 189#8211;198 [14] L.J. Ren, G.N. Sun, X.J. Ji, X.C. Hu, H. Huang, Compositional shift in lipid fractions during lipid accumulation and turnover in Schizochytrium sp, Bioresour. Technol. 157 (2014) 107#8211;113. [15] A.C. Guedes, H.M.Amaro,M.S. Giatilde;o, F.X. Malcata, Optimization of ABTS radical cation assay specifically for determination of antioxidant capacity of intracellular extractsof microalgae and cyanobacteria, Food Chem. 138 (1) (2013) 638#8211;643. |
3. 毕业设计(论文)进程安排
起讫日期 |
设计(论文)各阶段工作内容 |
备 注 |
12.30-01.30 |
查阅文献,完成开题报告 |
|
01.30#8212;03.01 |
完成英文翻译 |
|
03.01-05.30 |
完成实验工作 |
|
06.01-06.10 |
完成毕业论文 |
|