1. 毕业设计（论文）的内容和要求

1．文献查阅 掌握文献查阅的一般方法，学会使用计算机在中国期刊网，维普数据库，超星数字图书馆，美国化学学会（acs）数据库，ca，sci等检索资源上查阅关于搅拌桨组合及cfd在发酵中应用的相关文献。

2．文献阅读及综述 阅读与课题相关的外文及中文文献，了解国内外的研究动态，翻译一篇英文文献，提交一篇开题报告。

格式要求为：参考文献标注符合规范。

2. 参考文献

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Effect of poly (gamma-glutamic acid) on wheat productivity, nitrogen use efficiency and soil microbes[J]. JOURNAL OF SOIL SCIENCE AND PLANT NUTRITION, 2013,13(3):744~755. [7] Bajaj I B, Singhal R S. Sequential Optimization Approach for Enhanced Production of Poly(gamma-Glutamic Acid) from Newly Isolated Bacillus subtilis[J]. FOOD TECHNOLOGY AND BIOTECHNOLOGY, 2009,47(3):313~322. [8] Du G C, Yang G, Qu Y B, et al. Effects of glycerol on the production of poly(gamma-glutamic acid) by Bacillus licheniformis[J]. PROCESS BIOCHEMISTRY, 2005,40(6):2143~2147. [9] Yoon S H, Hwan Do J, Lee S Y, et al. Production of poly-gamma-glutamic acid by fed-batch culture of Bacillus licheniformis[J]. BIOTECHNOLOGY LETTERS, 2000,22(7):585~588. [10] Yao J, Xu H, Shi N, et al. Analysis of Carbon Metabolism and Improvement of γ-Polyglutamic Acid Production from Bacillus subtilis NX-2[J]. Applied Biochemistry and Biotechnology, 2010,160(8):2332~2341. [11] Yoon S H, Do J H, Lee S Y, et al. Production of poly-γ-glutamic acid by fed-batch culture of Bacillus licheniformis[J]. Biotechnology Letters, 2000,22(7):585~588. [12] Kunioka M. Biosynthesis of poly (γ-glutamic acid) from L-glutamine, citric acid and ammonium sulfate in Bacillus subtilis IFO3335[J]. Applied Microbiology and Biotechnology, 1995,44(3):501~506. [13] Bajaj I B, Singhal R S. Sequential Optimization Approach for Enhanced Production of Poly(gamma-Glutamic Acid) from Newly Isolated Bacillus subtilis[J]. FOOD TECHNOLOGY AND BIOTECHNOLOGY, 2009,47(3):313~322. [14] Bajaj I B, Singhal R S. Effect of aeration and agitation on synthesis of poly (γ-glutamic acid) in batch cultures of Bacillus licheniformis NCIM 2324[J]. Biotechnology and Bioprocess Engineering, 2010,15(4):635~640. [15] Xu H, Jiang M, Li H, et al. Efficient production of poly(γ-glutamic acid) by newly isolated Bacillus subtilis NX-2[J]. Process Biochemistry, 2005,40(2):519~523. [16] Zhang D, Feng X, Li S, et al. Effects of oxygen vectors on the synthesis and molecular weight of poly(γ-glutamic acid) and the metabolic characterization of Bacillus subtilis NX-2[J]. Process Biochemistry, 2012,47(12):2103~2109. [17] Zhang D, Xu Z, Xu H, et al. Improvement of poly(γ-glutamic acid) biosynthesis and quantitative metabolic flux analysis of a two-stage strategy for agitation speed control in the culture of Bacillus subtilis NX-2[J]. Biotechnology and Bioprocess Engineering, 2011,16(6):1144~1151. [18] 汤宝, 冯小海, 张丹, 等. 通过透明颤菌血红蛋白基因表达提高γ-聚谷氨酸的生物合成[J]. 生物加工过程, 2016(02):1~6. [19] Kumar R, Pal P. Fermentative production of poly (γ-glutamic acid) from renewable carbon source and downstream purification through a continuous membrane-integrated hybrid process[J]. Bioresource Technology, 2015,177:141~148. [20] Wei X, Tian G, Ji Z, et al. A new strategy for enhancement of poly-γ-glutamic acid production by multiple physicochemical stresses in Bacillus licheniformis[J]. Journal of Chemical Technology Biotechnology, 2015,90(4):709~713. [21] Xu Z, Feng X, Zhang D, et al. Enhanced poly(γ-glutamic acid) fermentation by Bacillus subtilis NX-2 immobilized in an aerobic plant fibrous-bed bioreactor[J]. Bioresource Technology, 2014,155:8~14. [22] Yang N, Wu Z, Chen J, et al. Multi-scale analysis of gas#8211;liquid interaction and CFD simulation of gas#8211;liquid flow in bubble columns[J]. Chemical Engineering Science, 2011,66(14):3212~3222. [23] 李艳, 詹晓北, 郑志永. 新型搅拌桨组合在非牛顿流体中的传氧性能研究[J]. 食品与机械, 2008(04):93~97. [24] Sardeing R, Aubin J, Xuereb C. Gas-liquid mass transfer - A comparison of down- and up-pumping axial flow impellers with radial impellers[J]. CHEMICAL ENGINEERING RESEARCH DESIGN, 2004,82(A12):1589~1596. [25] 郝志刚, 包雨云, 高正明. 多层组合桨搅拌槽内气-液分散特性的研究[J]. 高校化学工程学报, 2004(05):547~552. [26] Cai M, Zhou X, Lu J, et al. Enhancing aspergiolide A production from a shear-sensitive and easy-foaming marine-derived filamentous fungus Aspergillus glaucus by oxygen carrier addition and impeller combination in a bioreactor[J]. Bioresource Technology, 2011,102(3):3584~3586. [27] Xia J, Wang Y, Zhang S, et al. Fluid dynamics investigation of variant impeller combinations by simulation and fermentation experiment[J]. Biochemical Engineering Journal, 2009,43(3):252~260. [28] Yang Y, Xia J, Li J, et al. A novel impeller configuration to improve fungal physiology performance and energy conservation for cephalosporin C production[J]. Journal of Biotechnology, 2012,161(3):250~256. [29] Alfaro-Ayala J A, Ayala-Ram#237;rez V, Gallegos-Mu#241;oz A, et al. Optimal location of axial impellers in a stirred tank applying evolutionary programing and CFD[J]. Chemical Engineering Research and Design, 2015,100:203~211. [30] Taghavi M, Zadghaffari R, Moghaddas J, et al. Experimental and CFD investigation of power consumption in a dual Rushton turbine stirred tank[J]. Chemical Engineering Research and Design, 2011,89(3):280~290. [31] 李良超, 王嘉骏, 顾雪萍, 等. 气液搅拌槽内气泡尺寸与局部气含率的CFD模拟[J]. 浙江大学学报(工学版), 2010(12):2396~2400. [32] 吴学凤, 姜绍通, 张旻, 等. 机械搅拌生物反应器的CFD模拟及其在发酵生产乳酸中的应用[J]. 食品科学, 2010(07):186~189. [33] Ahmed S U, Ranganathan P, Pandey A, et al. Computational fluid dynamics modeling of gas dispersion in multi impeller bioreactor[J]. Journal of Bioscience and Bioengineering, 2010,109(6):588~597. [34] 董淑浩, 朱萍, 徐晓滢, 等. 高粘发酵体系不同搅拌桨的CFD模拟及发酵验证[J]. 生物工程学报, 2015(07):1099~1107.

3. 毕业设计（论文）进程安排

起讫日期 设计（论文）各阶段工作内容 备 注 2015.10.10#8212;2015.10.29 确定论文题目、填写任务书 2015.10.30--2015.11.06 毕业设计（论文）任务书及进度表 2015.11.07--2015.12.06 查找资料、写开题报告 2015.12.07--2016.01.20 初步实验及验证 2016.03.01--2016.03.30 完善实验、数据处理 2016.04.01--2016.04.15 论文写作 2016.04.16--2016.04.25 完成论文初稿交老师批阅 2016.04.26--2016.05.15 论文修改 2016.05.16#8212;2016.06.01 论文答辩准备