1. 毕业设计（论文）的内容和要求

一、论文内容

建立杀菌剂三氯生在莱茵衣藻体内高效液相检测方法，并通过三氯生在莱茵衣藻体内代谢物的检测推测其代谢途径。具体内容如下：

1、建立莱茵衣藻中三氯生的液相检测方法；

2. 参考文献

[1] ye x, bishop a m, needham l l, et al. automated on-line column-switching hplc-ms/ms method with peak focusing for measuring parabens, triclosan, and other environmental phenols in human milk[j]. analytica chimica acta, 2008, 622(1): 150-156.

[2] piccoli a, fiori j, andrisano v, et al. determination of triclosan in personal health care products by liquid chromatography (hplc)[j]. il farmaco, 2002, 57(5): 369-372.

[3] sun k, kang f, waigi m g, et al. laccase-mediated transformation of triclosan in aqueous solution with metal cations and humic acid[j]. environmental pollution, 2017, 220: 105-111.

3. 毕业设计（论文）进程安排

2016.12-2017.01：文献检索，英文论文翻译；

2017.01-2017.03：完成三氯生液相检测方法的建立与不同浓度三氯生在莱茵衣藻体内积累量测定

2017.03-2017.04：完成莱茵衣藻体内三氯生代谢产物鉴定并推测代谢途径；