1. 毕业设计（论文）的内容和要求

1．文献查阅 掌握文献查阅的一般方法，学会使用计算机在中国期刊网，维普数据库，超星数字图书馆，elsevier、ncbi电子期刊，springer link全文电子期刊，pubmed等检索资源上查阅关于遗传转化体系构建的相关文献。

2．文献阅读及综述 阅读与课题相关的外文及中文文献，了解国内外的研究动态，撰写文献综述。

3．明确实验任务，拟定实验方案 根据所查阅文献的内容，确定实验内容及方案，拟定科学可行的研究计划。

2. 参考文献

1. Li H, Zhu D, Hyatt B A. Cloning, Protein Sequence Clarification, and Substrate Specificity of a Leucine Dehydrogenase from Bacillus sphaericus ATCC4525[J]. Applied Biochemistry and Biotechnology, 2009, 158(2):343-51. 2. Li J, Pan J, Zhang J, et al. Stereoselective synthesis of l - tert -leucine by a newly cloned leucine dehydrogenase from Exiguobacterium sibiricum[J]. Journal of Molecular Catalysis B Enzymatic, 2014, 105(7):11-17. 3. Ohshima T, Nishida N, Bakthavatsalam S, et al. The purification, characterization, cloning and sequencing of the gene for a halostable and thermostable leucine dehydrogenase from Thermoactinomyces intermedius.[J]. European Journal of Biochemistry, 1994, 222(2):305-12. 4. Lee M, Kim J H, Lee S H, et al. Biomimetic artificial photosynthesis by light-harvesting synthetic wood.[J]. Chemsuschem, 2011, 4(5):581-586. 5. Lee S H, Ryu J, Nam D H, et al. Photoenzymatic synthesis through sustainable NADH regeneration by SiO2-supported quantum dots.[J]. Chemical communications (Cambridge, England), 2011, 47(16):4643-5. 6. Zhong C, Gurry T, Cheng A A, et al. Self-Assembling Multi-Component Nanofibers for Strong Bioinspired Underwater Adhesives[J]. Nature Nanotechnology, 2014, 9(10):858-866. 7. Anika S, Mostaert SPJ. In: The Functional Fold: Amyloid Structures in Nature. Mostaert SJA, editor. Pan Stanford: 2012. pp. 131#8211;146. 8. Knowles TPJ, Oppenheim TW, Buell AK, Chirgadze DY, Welland ME. Nanostructured films from hierarchical self-assembly of amyloidogenic proteins. Nature Nanotechnology. 2010;5:204#8211;207. 9. Goulter-Thorsen R, Taran E, Gentle I, Gobius K, Dykes G. CsgA production by Escherichia coli O157: H7 alters attachment to abiotic surfaces in some growth environments. Applied and environmental microbiology. 2011;77:7339#8211;7344. 10. Botyanszki Z, Tay P K, Nguyen P Q, et al. Engineered catalytic biofilms: Site-specific enzyme immobilization onto E. coli curli nanofibers.[J]. Biotechnology and Bioengineering, 2015, 112(10):96.

3. 毕业设计（论文）进程安排

起讫日期 设计（论文）各阶段工作内容 备 注 2017.1.2-2017.1.19 文献查询和资料阅读 2017.1.19-2017.1.23 研究方案制定和开题报告写作 2017.1.24-2017.3.10 实践内容的准备 2017.3.11-2017.5.10 实践 2017.5.10-2017.5.15 实践内容处理与结果分析 2017.5.16-2017.6.1 毕业论文书写