1. 毕业设计（论文）的内容和要求

天然酶存在于自然就界中，由于其具有较高的催化效率及基底特异性而被广泛应用于各领域。

然而由于大多数天然酶都是蛋白质，在非生理条件下容易失活，且在生物体内含量很低，分离纯化较困难，因而价格昂贵。

为了提高酶的稳定性并降低成本，开发新型的酶替代物成为了研究的热点。

2. 参考文献

[1] Junyu Lu, Yuhao Xiong, Chunjin Liao and Fanggui Ye* .Colorimetric detection of uric acid in human urine and serum based on peroxidase mimetic activity of MIL-53(Fe) [J] .Anal.Methods, 2015,7, 9894. [2] Zhu X., Zheng H., Wei X., et al. Metal-organic framework (MOF): a novel sensing platform for biomolecules. Chem. Commun., 2013, 49: 1276-1278. [3] Suh M. P., Park H. J., Prasad T. K., et al. Hydrogen storage in metal-organic frameworks.Chem. Rev., 2012, 112: 782-835. [4] Li J. R., Sculley J., Zhou H. C. Metal-organic frameworks for separations. Chem. Rev.,2012, 112: 869-932. [5] Horcajada P., Gref R., Baati T., et al. Metal-organic frameworks in biomedicine. Chem.Rev., 2012, 112: 1232-1268. [6] Lee J., Farha O. K., Roberts J., et al. Metal-organic framework materials as catalysts.Chem. Soc. Rev., 2009, 38: 1450-1459. [7] Allendorf M. D., Bauer C. A., Bhakta R. K., et al. Luminescent metal-organic frameworks. Chem. Soc. Rev., 2009, 38: 1330-1352. [8] Kreno L. E., Leong K., Farha O. K., et al. Metal-organic framework materials as chemical sensors. Chem. Rev., 2012, 112: 1105-1125. [9] Chen Y.-Q., Li G.-R., Chang Z., et al. A Cu (I) metal-organic framework with 4-fold helical channels for sensing anions. Chem. Sci., 2013, 4: 3678-3682. [10] Ai L., Li L., Zhang C., et al. MIL-53 (Fe): a metal-organic framework with intrinsic peroxidase-like catalytic activity for colorimetric biosensing. Chem. Eur. J., 2013, 19:15105-15108. [11] Zhiming Yan,a Wenmin Zhang,a Jia Gao,a Yifen Lin,a Jianrong Li,c Zian Lin*a and Lan Zhang*ab .Reverse-phase high performance liquid chromatography separation of positional isomers on a MIL-53(Fe) packed column?[J] .RSC Adv., 2015,5, 40094. [12] Mustafa Salih Hizir,?Meryem Top,?Mustafa Balcioglu,?Muhit Rana,?Neil M. Robertson,?Fusheng Shen,?Jia Sheng,??and Mehmet V. Yigit* .Multiplexed Activity of perAuxidase: DNA-Capped AuNPs Act as Adjustable Peroxidase [J] .Chem. 2016, 88, 600#8722;605. [13] Biwu Liu and Juewen Liu. Accelerating peroxidase mimicking nanozymes using DNA?[J] . Nanoscale, 2015, 7, 13831. [14] Pengjuan Ni,Yujing Sun,Wangdong Lu,Yinlin wang,Zhen Li,Zhuang Li .Colorimetric assay for acetylcholinesterase and inhibitor screening based on the Ag[I]ion-3,3',5,5'-tetramethylbenzi -dine(TMB) [J] .Sensors and Actuators B 226(2016)104-109. [15] Xiang Zhang,Xueqing Bi,Weihua Di,Weiping Qin .A simple and sensitive Ce(OH)CO3/H2O2/ TMB reaction system for colorimetric determination of H2O2 and glucose [J] . Sensors and Actuators B 231(2016)714-722. [16] Nagarkar S. S., Joarder B., Chaudhari A. K., et al. Highly selective detection of nitro explosives by a luminescent metal-organic framework. Angew. Chem. Int. Ed., 2013, 52: 2881-2885. [17] Chen L., Zheng H., Zhu X., et al. Metal-organic frameworks-based biosensor for sequence-specific recognition of double-stranded DNA. Analyst, 2013, 138: 3490. [18] Fang J. M., Leng F., Zhao X. J., et al. Metal-organic framework MIL-101 as a low background signal platform for label-free DNA detection. Analyst, 2014, 139: 801-806. [19] Wu Y., Han J., Xue P., et al. Nano metal-organic framework (NMOF)-based strategies for multiplexed microRNA detection in solution and living cancer cells. Nanoscale, 2015, 7: 1753-1759.

3. 毕业设计（论文）进程安排

2017.1.2-2017.3.1 查阅资料，完成开题报告和任务书 2017.3.2-2017.3.10 确定方案，学习实验过程和操作 2017.3.11-2017.4.30 独立实验，合成材料并进行结构表征 2017.5.1-2017.5.30 数据整理，书写论文，制作PPT 2017.5.30- 修改论文并完成答辩