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毕业论文网 > 任务书 > 化学化工与生命科学类 > 化学 > 正文

二氧化钛纳米线作荧光猝灭剂构建检测核酸的生物传感器任务书

 2020-06-08 21:18:41  

1. 毕业设计(论文)的内容和要求

课题研究的内容: 生物体内活性分子(如:核酸、酶、蛋白质)的分析与检测,对获取生命过程中的化学与生物信息、了解生物分子的结构与功能关系、阐述生命活动的机理以及疾病的诊断都具有重要的意义。

新的分子生物学技术不断涌现,并在科学研究领域和实际应用中得到不断的发展与完善,使生物分子检测的灵敏度和特异性都得到很大的提高。

然而,随着疾病诊断要求的不断提高与科学研究的不断深入,我们迫切需要发展一些灵敏度高、选择性好、操作简单且成本低廉的生物传感器以便更好地满足科学研究和实际应用的需求。

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2. 参考文献

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One-pot synthesized DNA-CdTe quantum dots applied in a biosensor for the detection of sequence-specific oligonucleotides. Chem. Eur. J., 2012, 18: 8296-8300. [12] Obliosca J. M., Liu C., Batson R. A., et al. DNA/RNA detection using DNA-templated few-atom silver nanoclusters. Biosensors, 2013, 3: 185-200. [13] Liu J. DNA-stabilized, fluorescent, metal nanoclusters for biosensor development. TrAC, Trends Anal. Chem., 2014, 58: 99-111. [14] Li S., Cao W., Kumar A., et al. Highly sensitive simultaneous detection of mercury and copper ions by ultrasmall fluorescent DNA-Ag nanoclusters. New J. Chem., 2014, 38: 1546-1550. [15] Deng L., Zhou Z., Li J., et al. Fluorescent silver nanoclusters in hybridized DNA duplexes for the turn-on detection of Hg2 ions. Chem. Commun., 2011, 47: 11065-11067. [16] Obliosca J. M., Liu C., Yeh H.-C. Fluorescent silver nanoclusters as DNA probes. Nanoscale, 2013, 5: 8443-8461. [17] Huang D., Niu C., Wang X., et al. Turn-On fluorescent sensor for Hg2 based on single-stranded DNA functionalized Mn:CdS/ZnS quantum dots and gold nanoparticles by time-gated mode. Anal. Chem., 2013, 85: 1164-1170. [18] Piao Y., Liu F., Seo T. S. A novel molecular beacon bearing a graphite nanoparticle as a nanoquencher for in situ mRNA detection in cancer cells. ACS Appl. Mater. Interfaces, 2012, 4: 6785-6789. [19] Qian Z., Shan X., Chai L., et al. Simultaneous detection of multiple DNA targets by integrating dual-color graphene quantum dot nanoprobes and carbon nanotubes. Chem. Eur. J., 2014, 20: 16065-16069. [20] Qian Z. S., Shan X. Y., Chai L. J., et al. Dual-colored graphene quantum dotslabeled nanoprobes/graphene oxide: functional carbon materials for respective and simultaneous detection of DNA and thrombin. Nanotechnology, 2014, 25: 1-10. [21] Qian Z. S., Shan X. Y., Chai L. J., et al. DNA nanosensor based on biocompatible graphene quantum dots and carbon nanotubes. Biosens. Bioelectron., 2014, 60: 64-70. [22] Zhang L., Xiao S. J., Zheng L. L., et al. Aptamer-mediated nanocomposites of semiconductor quantum dots and graphene oxide as well as their applications in intracellular imaging and targeted drug delivery. J. Mater. Chem. B, 2014, 2: 8558-8565. [23] Tu Y., Wu P., Zhang H., et al. Fluorescence quenching of gold nanoparticles integrating with a conformation-switched hairpin oligonucleotide probe for microRNA detection. Chem. Commun., 2012, 48: 10718-10720. [24] Zhang L., Li T., Li B., et al. Carbon nanotube#8211;DNA hybrid fluorescent sensor for sensitive and selective detection of mercury (ii) ion. Chem. Commun., 2010, 46: 1476-1478. [25] Qiang W., Li W., Li X., et al. Bioinspired polydopamine nanospheres: a superquencher for fluorescence sensing of biomolecules. Chem. Sci., 2014, 5: 3018-3024. [26] Zhang L., Guo S., Dong S., et al. Pd nanowires as new biosensing materials for magnified fluorescent detection of nucleic acid. Anal. Chem., 2012, 84: 3568-3573. 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3. 毕业设计(论文)进程安排

2017.1.2-2017.3.1 查阅资料,完成开题报告和任务书 2017.3.2-2017.3.10 确定方案,学习实验过程和操作 2017.3.11-2017.4.30 独立实验,合成材料并进行结构表征 2017.5.1-2017.5.30 数据整理,书写论文,制作PPT 2017.5.30- 修改论文并完成答辩

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