1. 毕业设计（论文）的内容和要求

1． 设计的内容 本研究通过考察不同抗氧化剂添加的效果，找到最优的抗氧化剂的添加品种，添加量及添加时间，提高纽莫康定b0发酵产量的同时，探究抗氧化剂作用原理。

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2． 要求 1) 温习各种查阅文献的方法，在此基础上阅读大量与课题相关资料（包括适量的外文文献），及时了解国内外有关的研究进展，为以后的实验打下基础。

2. 参考文献

[1] Denning, D.W., Echinocandin antifungal drugs [J]. Lancet, 2003. 362(9390): 1142-51. [2] Schwartz, R.E., et al., L-671,329, a new antifungal agent. I. Fermentation and isolation [J]. J Antibiot (Tokyo), 1989. 42(2): 163-7. [3] Bills, G.F., et al., Reclassification of a pneumocandin-producing anamorph, Glarea lozoyensis gen. et sp. nov. [J], previously identified as Zalerion arboricola. Mycological Research, 1999. 103: 179-192. [4] Hoffmeister D, Keller NP. Natural products of filamentous fungi: enzymes, genes, and their regulation [J]. Nat Prod Rep, 2007;24:393-416. [5] Brakhage AA, Thon M, Sprote P, Scharf DH, Al-Abdallah Q, Wolke SM, et al. Aspects on evolution of fungal beta-lactam biosynthesis gene clusters and recruitment of trans-acting factors [J]. Phytochemistry, 2009, 70:1801-11. [6] Brakhage AA, Schroeckh V. Fungal secondary metabolites - Strategies to activate silent gene clusters l[J]. Fungal Genet Bio, 2011, 48:15-22. [7] Richardt A, Kemme T, Wagner S, et al. Ebony,a novel nonribosomal peptide synthetase for bet a-alanine conjugation with biogenic amines in Drosophila [J]. J Biol Chem, 2003, 278:41160-41166. [8] Grunewald J,Marahiel MA. Chemoe nzymatic and templete directed synthesis of bioactive macrocyclic pepttide.Microbiol [J]. Mol Biol Rev,2006,7(1):121-146 [9] Mannanov RN, Sattarova RK. Chemistry of Nature Compounds, 2001, 37(2): 117~123. [10] Bradford MM. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding [J]. Analytical Biochemistry. 1976, 72: 248-254. [11] Suzuki T, Sato M, Yoshida T, et al. Rat liver mitochondrial and cytosolic fumarases with identical amino acid sequences are encoded from a single gene [J]. The Journal of Biological Chemistry, 1984, 264(5): 2581~2586. [12] Tomoko G,Shoji W, Hachiro O. Purification and Characterization of Fumarase from Corynebacterium glutamicum[J]. Biosci.Biotechnol.Biochem, 2006, 70 (5):1102~1109. [13] Kinsella, S. Doonan. Nucleotide sequence of a cDNA coding for mitochondrial fumarase from human liver [J]. Biosci Rep, 1986, 6: 921~929. [14] Kobayashi, K, T. Yamanishi. Physicochemical, catalytic, and immunochemical properties of fumarases crystallized separately from mitochondrial and cytosolic fractions of rat liver [J]. J Biochem, 1981, 89: 1923~1931. [15] Weaver TM, Lees M, Zaitsev V, et al. Crystal structures of native and recombinant yeast fumarase [J]. Journal of Molecular Biology, 1998, 280: 431~442. [16] Chance B,Sies H,Boveris A.Hydroperoxide metabolism in manmmalian organs.Physiological Reviwes,1979,59(3):527-605. [17] Xu J X,Li X,Zhang Y X,et al.In:Lester P.eds.Mitochondrial respiratory chain:A Salf-defense system againse oxygen toxicity.Proceedings of the international symposium on natural antioxidants:molecular mechanisms and health effects.Champaign,Illinois:AOCS Press.1996,530-539. [18] Allen R G,Balin A K.Oxidative influnce on development and differentiation:An overview of a free radical theory of development.Free Radical BiologyMedicine,1989,6(6):631-661. [19] Skulachew V P,Role of uncoupled and non-coupled oxidations in maintenance of safey low levels of oxygen and its one-electron reductants.Quart Rev Biophys,1996,29(1):169-202.

3. 毕业设计（论文）进程安排

2017.12-2018.1 查阅相关文献资料，掌握各种实验仪器设备的使用，准备开题报告的撰写 2018.2-2018.3 考察不同抗氧化剂的添加的效果 2018.4-2018.5 考察氧化剂的不同添加量及添加时间 2018.5-2018.6 探究抗氧化剂作用原理，进行中期检查报告，整理实验数据，撰写毕业论文