1. 毕业设计（论文）的内容和要求

论文内容 大肠杆菌(escherichia coli)因结构简单、遗传学背景清晰、生长周期短、生长条件清楚等优势成为最为常用的宿主菌，广泛应用于重组蛋白的表达。

特别是，大肠杆菌高密度发酵实现了生物质的高效积累以获取外源目标蛋白。

本课题是在实验室前期构建获得表达糖基转移酶的重组大肠杆菌基础上，进一步研究该菌株高密度发酵产酶的过程。

2. 参考文献

[1] Dieter riesenberg. High-cell-density cultivation of Escherichia coli. [J]. Current Opinion in Biotechnology, 1991, 2(3): 380-384. [2] I voss, Steinb#252;chel, A. High cell density cultivation of Rhodococcus opacus for lipid production at a pilot-plant scale. [J]. Applied Microbiology and Biotechnology, 2001, 55(5): 547-555. [3] Wa johnston,M stewart,P lee,at al. Tracking the acetate threshold using DO-transient control during medium and high cell density cultivation of recombinant Escherichia coli in complex media.[J]. Biotechnology and bioengineering, 2003, 84(3): 23-314. [4] Lee chang kj, G dumsday, Pd nichols, at al. High cell density cultivation of a novel Aurantiochytrium sp. strain TC 20 in a fed-batch system using glycerol to produce feedstock for biodiesel and omega-3 oils.[J]. Applied microbiology and biotechnology, 2013, 97(15): 18-6907. [5] Restaino O F, Bhaskar U, Paul P, et al. High cell density cultivation of a recombinant E. coli strain expressing a key enzyme in bioengineered heparin production.[J]. Applied Microbiology Biotechnology, 2013, 97(9):3893-3900. [6] Agger T, Spohr A B, Nielsen J. α-Amylase production in high cell density submerged cultivation of Aspergillus oryzae, and A. nidulans. [J]. Applied Microbiology Biotechnology, 2001, 55(1):81-84. [7] Li Z, Zhang X, Tan T. Lactose-induced production of human soluble B lymphocyte stimulator (hsBLyS) in E. coli, with different culture strategies. [J]. Biotechnology Letters, 2006, 28(7):477-483. [8] Takaomi kobayashi, Karla tovar-carrillo. Fibroblast cell cultivation on wooden pulp cellulose hydrogels for cytocompatibility scaffold method. [J]. OMICS International, 2015, 6(10): 1-9. [9] Sh ramadhan,T matsui,K nakano,at al. High cell density cultivation of Pseudomonas putida strain HKT554 and its application for optically active sulfoxide production.[J]. Applied microbiology and biotechnology, 2013, 97(5): 7-1903. [10] Y zhang. Flow feld analysis and structure optimization of a three-phase separation hydrocylones based on computational fluid dynamics (CFD). [J]. Chemical engineering machinery. 2010, 37(6): 744-749.

3. 毕业设计（论文）进程安排

2018.2.26-2018.3.2 学习基本实验操作，为后期实验打基础； 2018.3.3-2018.3.9 查阅文献，并结合预实验结果分析，合理设计实验方案； 2018.3.10-2018.4.20 研究优化摇瓶培养工程菌生长及产酶的条件； 2018.4.21-2018.5.31 研究优化5L发酵罐高密度发酵及产酶的条件； 2018.6.1-2018.6.15 及时撰写毕业论文。