1. 毕业设计（论文）的内容和要求

1．文献查阅 掌握文献查阅，使用网络资源如中国期刊网、维普数据库、超星数字图书馆、elsevier电子期刊、springer link全文电子期刊等检索资源，查阅关于甲醇利用方面的相关文献。

2．文献阅读及综述 阅读与课题相关的外文与中文文献，了解国内外的研究动态，撰写文献综述。

3．明确实验任务，拟定实验方案 根据所查阅文献的内容，确定实验内容及方案，拟定科学可行的研究计划。

2. 参考文献

1.Krog A, Heggeset TM, Ellingsen TE, Brautaset T (2013a) Functional characterization of key enzymes involved in L-glutamate synthesis and degradation in the thermotolerant and methylotrophic bacterium Bacillus methanolicus. Appl Environ Microbiol 79:5321#8211;5328. doi: 10.1128/AEM. 01382-13 2.Krog A, Heggeset TM, Muller JE, Kupper CE, Schneider O, Vorholt JA,Ellingsen TE, Brautaset T (2013b) Methylotrophic Bacillus methanolicus encodes two chromosomal and one plasmid born NAD dependent methanol dehydrogenase paralogs with different catalytic and biochemical properties. PLoS One 8:e59188. doi:10. 1371/journal.pone.0059188 3.Markert B, Stolzenberger J, Brautaset T, Wendisch VF (2014)Characterization of two transketolases encoded on the chromosome and the plasmid pBM19 of the facultative ribulose monophosphate cycle methylotroph Bacillus methanolicus. BMC Microbiol 14:7. doi:10.1186/1471-2180-14-7 4.Mills DA, Flickinger MC (1993) Cloning and sequence analysis of the meso-diaminopimelate decarboxylase gene from Bacillus methanolicus MGA3 and comparison to other decarboxylase genes. Appl Environ Microbiol 59:2927#8211;2937 5.M#252;ller JE, Litsanov B, Bortfeld-Miller M, Trachsel C, Grossmann J, Brautaset T, Vorholt JA (2014) Proteomic analysis of the thermophilic methylotroph Bacillus methanolicus MGA3. Proteomics 14:725#8211;737. doi:10.1002/pmic.201300515 6.N#230;rdal I, Netzer R, Ellingsen TE, Brautaset T (2011) Analysis and manipulation of aspartate pathway genes for L-lysine overproduction from methanol by Bacillus methanolicus. Appl Environ Microbiol 77:6020#8211;6026. doi:10.1128/AEM. 05093-11 7.N#230;rdal I, Pfeifenschneider J, Brautaset T, Wendisch VF (2014) Methanol-based cadaverine production by genetically engineered Bacillus methanolicus strains. submitted 8.Nguyen HD, Nguyen QA, Ferreira RC, Ferreira LC, Tran LT, Schumann W (2005) Construction of plasmid-based expression vectors for Bacillus subtilis exhibiting full structural stability. Plasmid 54: 241#8211;248. doi:10.1016/j.plasmid.2005.05.001

3. 毕业设计（论文）进程安排

2017.12-2018.2 文献查阅，完成开题报告，熟悉实验仪器。

2018.3-2018.4 熟悉微生物操作，进行实验,取样 2018.4-2018.5 多次传代，纯化菌株，得到耐高温的嗜甲基营养菌 2018.5-2018.6 进行菌种鉴定，并分析代谢产物