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毕业论文网 > 任务书 > 化学化工与生命科学类 > 食品质量与安全 > 正文

基于磁性Fe3O4-壳聚糖纳米颗粒固定化酪丁酸梭菌以促进丁酸的高产任务书

 2020-04-16 16:43:33  

1. 毕业设计(论文)的内容和要求

内容 基于磁性Fe3O4-壳聚糖纳米颗粒固定化酪丁酸梭菌以促进丁酸的高产,利用共沉淀法制备磁性纳米颗粒,然后采用戊二醛交联法制备Fe3O4-壳聚糖载体,采用制备好的磁性Fe3O4-壳聚糖纳米颗粒固定化酪丁酸梭菌进行发酵。

要求 1.掌握一定的微生物学知识,能够独立完成酪丁酸梭菌产丁酸的发酵;2.掌握一定的物理和化学知识,能够独立制备载体;3.能够使用origin数据处理软件。

2. 参考文献

[1] Jin Huang, Wan Tang, et al. Production of butyric acid by Clostridium tyrobutyricum coated with carboxyl functioned magnetic nanoparticles[J]. Chemical Technology and Biotechnology, 2018, 93(8):2364-2370. [2] Juanjuan Guo, Zhichang Zheng, et al. Enhanced Production of κ#8209;Carrageenase and κ#8209;Carrageenan Oligosaccharides through Immobilization of Thalassospira sp. Fjfst-332 with Magnetic Fe3O4#8209;Chitosan Microsphere[J]. Agricultural and Food Chemistry, 2017, 65, 7934-7943. [3] Guanyi Chen, Jing Liu, et al. Biodiesel production using magnetic whole-cell biocatalysts by immobilization of Pseudomonas mendocina on Fe304-chitosan microspheres[J]. Biochemical Engineering, 2016,113,86-92. [4] Guiyin Li, Yuren Jiang, et al. Preparation and properties of magnetic Fe3O4-chitosan nanoparticles[J]. Alloys and Compounds, 2008,466 ,451#8211;456. [5] Yong Liu, Shaoyi Jia, et al. Studies of Fe3O4-chitosan nanoparticles prepared by co-precipitation under the magnetic #64257;eld for lipase immobilization[J]. Catalysis Communications,2011, 12 , 717#8211;720. [6] J. M. D. Coey. Magnetism and magnetic materials[M]. 北京:北京大学出版社,2014. [7]陈立钢等.磁性纳米复合材料的制备与应用[M]. 北京:科学出版社,2016. [8]匡少平,吴占超.磁性壳聚糖纳米材料的制备与应用[M]. 北京:化学工业出版社,2017. [9] Jie Long,Xingfei Li, et al. Immobilization of pullulanase onto activated magnetic chitosan/Fe3O4 nanoparticles prepared by in situ mineralization and effect of surface functional groups on the stability[J]. Colloids and Surfaces A: Physicochemical and Engineering Aspects,2015, 472,69-77. [10] 朱广谦,何青科等.Fe3O4磁性纳米粒子的制备及其表面修饰[J].湖南师范大学自然科学学报,2016,39(03):46-55 74. [11] Ling Jiang, Jufang Wang et al. Production of Butyric Acid from Glucose and Xylose with Immobilized Cells of Clostridium tyrobutyricum in a Fibrous-bed Bioreactor [J]. Applied biochemistry and biotechnology, 2010 ,160(2) ,350-360. [12] 高振,江凌等.纤维床固定化酪丁酸梭菌发酵木薯渣水解液生产丁酸的研究[J].安徽农业科学,2012,40(33):16302-16306. [13]陈佳璐,陈国等.羧基修饰的超顺磁纳米粒子直接固定化罗伊氏乳杆菌[J].化工学报,2012,63(04):1175-1181. [14]孙俊. 功能性磁性纳米颗粒的制备及其在食品体系中的应用研究[D].江南大学,2012. [15]胡晓龙,李聪聪等.酪丁酸梭菌RL1产丁酸发酵条件优化研究[J].轻工学报,2018,33(04):21-28. [16] Jie Zhang, Wenming Zong, et al. Exploiting endogenous CRISPR-Cas system for multiplex genome editing in Clostridium tyrobutyricum and engineer the strain for high-level butanol production[J], Metabolic Engineering, 2018, 47:49-59. [17]阳静璇. 羟基化合物修饰四氧化三铁纳米颗粒的研究[D].电子科技大学,2017. [18]项宜娟. 纤维床固定化酪丁酸梭菌产丁酸的研究[D].浙江理工大学,2010. [19] S#243;crates Palacios-Ponce, Rodolfo Ramos-Gonz#225;lez, et al. Trichoderma sp. spores and Kluyveromyces marxianus cells magnetic separation: Immobilization on chitosan-coated magnetic nanoparticles [J]. Preparative Biochemistry and Biotechnology,2017,47(06):554-561. [20] 靳艳艳,程武等.单分散羧基化Fe3O4磁性纳米粒子的制备及表征[J].科学通报,2014,59(18):1700-1706. [21] Dwidar M, Park JY, Mitchell RJ and Sang BI, The future of butyric acid in industry. Sci World J 2012:471417 (2012).

3. 毕业设计(论文)进程安排

2018年11月:确定选题,查阅资料,开题,拟定总体实验方案; 2018年12月:制备磁性Fe3O4-壳聚糖纳米颗粒作为载体; 2019年1月-2月:载体固定化酪丁酸梭菌以及固定化影响因素的探究; 2019年3月-4月:载体固定化酪丁酸梭菌产丁酸发酵实验; 2019年5月:完成毕业论文编写; 2019年6月:准备答辩。

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