1. 毕业设计（论文）的内容和要求

查阅相关文献及专业书籍了解目前荧光探针的发展及其对金属离子检测的现状研究，了解已有成果以及存在的问题，对论文的研究方向有较深入的认识。

采取合适的实验步骤，适宜的实验条件，合成并纯化目标产物l-1；将l-1用于检测不同的金属离子，观察荧光的变化并记录。

培养学生独立思考及动手做实验的能力，养成良好的科研习惯。

2. 参考文献

[1] Xiang Y, Tong A, Jin P, et al. New Fluorescent Rhodamine Hydrazone Chemosensor for Cu(II) with High Selectivity and Sensitivity[J]. Organic Letters, 2006,8(13):2863-2866. [2] 袁跃华, 殷强锋, 冯锋, 等. 新罗丹明类荧光探针的合成及对铜(Ⅱ)的检测[J]. 分析试验室, 2018,37(04):373-377. [3] 王鹏. 新型罗丹明B-色氨酸荧光探针合成及用于铜离子检测研究[J]. 西华师范大学学报(自然科学版), 2018,39(04):390-394. [4] Goldberg J M, Wang F, Sessler C D, et al. Photoactivatable Sensors for Detecting Mobile Zinc[J]. Journal of the American Chemical Society, 2018,140(6):2020-2023. [5] Yang Y, Yook K, Tae J. A Rhodamine-Based Fluorescent and Colorimetric Chemodosimeter for the Rapid Detection of Hg2 Ions in Aqueous Media[J]. Journal of the American Chemical Society, 2005,127(48):16760-16761. [6] Zeng L, Miller E W, Pralle A, et al. A Selective Turn-On Fluorescent Sensor for Imaging Copper in Living Cells[J]. Journal of the American Chemical Society, 2006,128(1):10-11. [7] Zhou Y, Wang F, Kim Y, et al. Cu2 -Selective Ratiometric and ”Off-On” Sensor Based on the Rhodamine Derivative Bearing Pyrene Group[J]. Organic Letters, 2009,11(19):4442-4445. [8] Ding Y, Zhu W, Xu Y, et al. A small molecular fluorescent sensor functionalized silica microsphere for detection and removal of mercury, cadmium, and lead ions in aqueous solutions[J]. Sensors and Actuators B: Chemical, 2015,220:762-771. [9] Gong X, Yang X, Zhong Y, et al. A flavylium-based turn-on fluorescent probe for imaging hydrogen polysulfides in living cells[J]. RSC Advances, 2016,6(91):88519-88525. [10] Chen X Q, Wang F, Hyun J Y, et al. Recent progress in the development of fluorescent, luminescent and colorimetric probes for detection of reactive oxygen and nitrogen species[J]. Chemical Society reviews, 2016,45(1):2316-2976.

3. 毕业设计（论文）进程安排

2019.02.23-03.15 查阅文献，完成翻译和开题报告 2019.03.12-04.25 熟悉实验技能，制备并纯化探针分子 2019.04.26-05.20 探针分子用于检测不同金属离子 2019.05.21-06.06 撰写毕业论文和答辩ppt