1. 毕业设计（论文）的内容和要求

本次论文的完成主要有以下要求：查阅相关文献资料，能够读懂英文文献；综合文献中的实验方法，设计与课题相关的条件实验；开展各个不同的条件实验，在实际操作过程中要能够分析并解决突发问题，能够对不同的实验结果进行分析对比；通过红外、核磁光谱对目标产物进行鉴定，能够对谱图进行分析；最后完成撰写本课题论文的任务。

2. 参考文献

[1] Bur J, Dekker M. Chemi- and Bio-luminescence [J]. Methods Enzymol, 1978,57-61 [2] Harvey E. Bioluminescence [M]. New York: Academic Press, 1952 [3] Harvey E. A History of Luminescence [M]. Philadelphia: The Academic Philosophical Society, 1957 [4] E. N. Harvey. The Nature of Animal Light. J. P. Lippincott Company. Philadelphia, 1920 [5] Babu B. G, Kannan M. Lightning Bugs. Resonance. 2002, 7:49-55 [6] Hastings J W. Chemistries and Color of Bioluminescent Reactions [J]. Gene, 1996, 173:5-11 [7] Deluca M. Transient and Stable Expression of the firefly luciferase gene in plant cells and transgenic plants [J]. Science, 1986, 234(4778):856-859 [8] Riss TL, Moravec RA. Use of multiple assay endpoints to investigate the effects of incubation time, dose of toxin, and plating density in cell-based cytotoxicity assays. Assay Drug Dev Technol, 2004, 2(1):51-62 [9] Lugons SK, Meuwissen R, Krimpenfort P, et al. The generation of a conditional reporter that enables bioluminescence imaging of Crelloxp-dependent tumorigenesis in mice [J]. Cancer Res, 2003, 63(21):7042-7046 [10] Brogan J, Li F, Li W, et al. Imaging molecular pathways: reporter genes[J]. Radiat Res, 2012, 177(4):508-513 [11] Nakamura M, et al. Tetrohedron let, 2006, 47. [12] 张菊梅, 吴清平, 周小燕等. 荧光素酶研究进展[J]. 微生物学通报, 2001, 28(5):98-101 [13] White E H, Worther H, Seliger H H, et al. Amino analogs of firefly luciferin and biological activity therofl [J]. Journal of the American chemical Society, 1966, 88(9):2015-2019

3. 毕业设计（论文）进程安排

2019.02.25-2019.03.15 查阅国内外有关文献，做文献综述；拟定实验方案。

2019.03.18-2019.05.17 按照实验要求进行各种实验工作。

2019.05.20-2019.05.31进行实验数据的处理，补充有关实验，开始撰写论文。