1. 毕业设计（论文）的内容和要求

随着越来越多的泛素修饰蛋白被报道，各种类泛素蛋白也不断被发现，如sumo(small ubiquitin-related modifier)、nedd8、atg8(autophagy gene 8)和atg12等。

目前，研究较多的为sumo和nedd8。

nedd8是由81个氨基酸残基组成的蛋白质，与泛素有60%的一致性和80%同源性。

2. 参考文献

[1]Teresa A. Soucy, Peter G. Smith, Michael A. Milhollen etc. (2009) An inhibitor of NEDD8-activating enzyme as a new approach to treat cancer. Nature, Vol 458:732-737. [2]Brownell J E, Sintchak M D, Gavin J M, et al. Substrate-Assisted Inhibition of Ubiquitin-like Protein-Activating Enzymes: The NEDD8 E1 Inhibitor MLN4924 Forms a NEDD8-AMP Mimetic In Situ[J]. Molecular Cell, 2010, 37(1):102#8211;111. [3]Tanaka T, Nakatani T, Kamitani T. Inhibition of NEDD8-conjugation pathway by novel molecules: Potential approaches to anticancer therapy[J]. Molecular Oncology, 2012, 6(3):267-275. [6]Yan Z H, Burkhardt A, Loke H K, et al. Quantifiable analysis of cellular pathway inhibition of a Nedd8-activating enzyme inhibitor, MLN4924, using AlphaScreen[J]. Analytical Biochemistry, 2013, 439(2):109-115. [4]Hjerpe R, Thomas Y, Kurz T. NEDD8 overexpression results in neddylation of ubiquitin substrates by the ubiquitin pathway.[J]. Journal of Molecular Biology, 2012, 421(1):27#8211;29.

3. 毕业设计（论文）进程安排

2019.2.25-2019.3.11 文献调研，撰写开题报告和试验方案 2019.3.12-2019.5.15 实验探索，完成化合物合成，获取实验数据 2019.5.16-2019.6.110 撰写毕业设计论文，完成毕业论文答辩