1. 毕业设计（论文）的内容和要求

1 文献阅读与归纳，深入了解课题背景 2 大肠杆菌重组菌的构建 3 耦联催化体系的构建 4 独立完成毕业论文的撰写

2. 参考文献

1 Motomura K, Hirota R, Mai O, et al. A New Subfamily of Polyphosphate Kinase 2 (Class III PPK2) Catalyzes both Nucleoside Monophosphate Phosphorylation and Nucleoside Diphosphate Phosphorylation[J]. Applied Environmental Microbiology, 2014, 80(8):2602-2608. 2 Akhtar M K, Turner N J, Jones P R. Carboxylic acid reductase is a versatile enzyme for the conversion of fatty acids into fuels and chemical commodities[J]. Proceedings of the National Academy of Sciences, 2013, 110(1): 87-92. 3 Liu S, Li Y, Zhu J. Enzymatic production of l-theanine by γ-glutamylmethylamide synthetase coupling with an ATP regeneration system based on polyphosphate kinase[J]. Process Biochemistry, 2016, 51(10): 1458-1463.

3. 毕业设计（论文）进程安排

2018.12-2019.01 阅读文献，完成开题报告 2019.03-2019.04 重组大肠杆菌的构建与验证 2019.05-2019.05 双酶耦联催化体系建立 2019.06-2019.06 撰写毕业论文