1. 毕业设计（论文）的内容和要求

1．文献查阅 掌握文献查阅的一般方法，学会使用计算机在中国期刊网、维普数据库、超星数字图书馆等中文数据库、ca、sci等外文数据库检索资源上查阅有关文献。

2．文献阅读及综述 阅读与课题相关的外文及中文文献，了解解脂耶氏酵母胞质中乙酰-coa含量对菌体产油脂和(-)-α-红没药醇情况的影响，撰写文献综述。

3．明确实验任务，拟定实验方案 根据所查阅文献的内容，确定实验内容及方案，拟定科学可行的研究计划。

2. 参考文献

Ajikumar, P.K., Xiao, W.-H., Tyo, K.E.J., Wang, Y., Simeon, F., Leonard, E., Mucha, O., Phon, T.H., Pfeifer, B., Stephanopoulos, G. 2010. Isoprenoid pathway optimization for Taxol precursor overproduction in Escherichia coli. Science (New York, N.Y.), 330(6000), 70-74. Attia, M., Kim, S., Ro, D. 2012. Molecular cloning and characterization of ( )-epi-α-bisabolol synthase, catalyzing the first step in the biosynthesis of the natural sweetener, hernandulcin, in Lippia dulcis. Arch. Biochem. Biophys., 527(1), 37-44. Becker, J., Wittmann, C. 2015. Advanced biotechnology: metabolically engineered cells for the bio-based production of chemicals and fuels, materials, and health-care products. Angew. Chem. Int. Ed. Engl., 54(11), 3328-50. Bouvier, F., Rahier, A., Camara, B. 2005. Biogenesis, molecular regulation and function of plant isoprenoids. Progress in lipid research, 44(6), 357-429. Cao, X., Lv, Y.B., Chen, J., Imanaka, T., Wei, L.J., Hua, Q. 2016. Metabolic engineering of oleaginous yeast Yarrowia lipolytica for limonene overproduction. Biotechnol Biofuels, 9, 214. Cao, X., Wei, L.J., Lin, J.Y., Hua, Q. 2017. Enhancing linalool production by engineering oleaginous yeast Yarrowia lipolytica. Bioresour Technol. Galanie, S., Thodey, K., Trenchard, I.J., Filsinger Interrante, M., Smolke, C.D. 2015. Complete biosynthesis of opioids in yeast. Science, 349(6252), 1095. Gao, S., Tong, Y., Zhu, L., Ge, M., Zhang, Y., Chen, D., Jiang, Y., Yang, S. 2017. Iterative integration of multiple-copy pathway genes in Yarrowia lipolytica for heterologous beta-carotene production. Metab Eng, 41, 192-201. Han, G.H., Kim, S.K., Yoon, P.K., Kang, Y., Kim, B.S., Fu, Y., Sung, B.H., Jung, H.C., Lee, D.H., Kim, S.W., Lee, S.G. 2016. Fermentative production and direct extraction of (-)-alpha-bisabolol in metabolically engineered Escherichia coli. Microb Cell Fact, 15(1), 185. Kildegaard, K.R., Adiego-Perez, B., Domenech Belda, D., Khangura, J.K., Holkenbrink, C., Borodina, I. 2017. Engineering of Yarrowia lipolytica for production of astaxanthin. Synth Syst Biotechnol, 2(4), 287-294. Li, J., Fang, X., Zhao, Q., Ruan, J., Yang, C., Wang, L., Miller, D., Faraldos, J., Allemann, R., Chen, X., Zhang, P. 2013. Rational engineering of plasticity residues of sesquiterpene synthases from Artemisia annua: product specificity and catalytic efficiency. Biochem. J., 451(3), 417-26. Liao, P., Hemmerlin, A., Bach, T.J., Chye, M.-L. 2016. The potential of the mevalonate pathway for enhanced isoprenoid production. Biotechnology advances, 34(5), 697-713. Nakano, C., Kudo, F., Eguchi, T., Ohnishi, Y. 2011. Genome mining reveals two novel bacterial sesquiterpene cyclases: (-)-germacradien-4-ol and (-)-epi-α-bisabolol synthases from Streptomyces citricolor. Chembiochem, 12(15), 2271-5. Nielsen, J., Keasling, Jay D. 2016. Engineering Cellular Metabolism. Cell, 164(6), 1185-1197. Paddon, C., Westfall, P.J., Pitera, D., Benjamin, K., Fisher, K., McPhee, D., D Leavell, M., Tai, A., Main, A., Eng, D., R Polichuk, D., H Teoh, K., Reed, D., Treynor, T., Lenihan, J., Fleck, M., Bajad, S., Dang, G., Diola, D., Newman, J. 2013. High-level semi-synthetic production of the potent antimalarial artemisinin. Roberts, S.C. 2007. Production and engineering of terpenoids in plant cell culture. Nature chemical biology, 3(7), 387-395. Rohdich, F., Hecht, S., G#228;rtner, K., Adam, P., Krieger, C., Amslinger, S., Arigoni, D., Bacher, A., Eisenreich, W. 2002. Studies on the nonmevalonate terpene biosynthetic pathway: Metabolic role of IspH (LytB) protein. Proceedings of the National Academy of Sciences, 99(3), 1158-1163. Son, Y.J., Kwon, M., Ro, D.K., Kim, S.U. 2014. Enantioselective microbial synthesis of the indigenous natural product (-)-alpha-bisabolol by a sesquiterpene synthase from chamomile (Matricaria recutita). Biochem J, 463(2), 239-48. Xue, Z., Sharpe, P.L., Hong, S.-P., Yadav, N.S., Xie, D., Short, D.R., Damude, H.G., Rupert, R.A., Seip, J.E., Wang, J., Pollak, D.W., Bostick, M.W., Bosak, M.D., Macool, D.J., Hollerbach, D.H., Zhang, H., Arcilla, D.M., Bledsoe, S.A., Croker, K., McCord, E.F., Tyreus, B.D., Jackson, E.N., Zhu, Q. 2013. Production of omega-3 eicosapentaenoic acid by metabolic engineering of Yarrowia lipolytica. Nature biotechnology, 31(8), 734-740. Yang, X., Nambou, K., Wei, L., Hua, Q. 2016. Heterologous production of α-farnesene in metabolically engineered strains of Yarrowia lipolytica. Bioresour Technol, 216(2016), 1040-8. Zhao, C., Gao, Q., Chen, J., Wei, L., Imanaka, T., Hua, Q. 2017. Metabolomic changes and metabolic responses to expression of heterologous biosynthetic genes for lycopene production in Yarrowia lipolytica. Journal of Biotechnology, 251, 174-185.

3. 毕业设计（论文）进程安排

1.10-3.1 确定毕业设计的课题及相关资料的查找 3.2-3.10 实验方案的设计 3.10-3.20 实验材料的准备 3.21-5.15 准备分子实验 5.15-6.5 实验数据处理与结果分析 5.20-6.10 毕业论文书写