1. 毕业设计（论文）的内容和要求

1．文献查阅 掌握文献查阅的一般方法，学会使用计算机在中国期刊网、维普数据库、超星数字图书馆等中文数据库、ca、sci等外文数据库检索资源上查阅有关文献。

2．文献阅读及综述 阅读与课题相关的外文及中文文献，了解糙皮侧耳发酵产麦角硫因的方法及过程，撰写文献综述。

3．明确实验任务，拟定实验方案 根据所查阅文献的内容，确定实验内容及方案，拟定科学可行的研究计划。

2. 参考文献

[1]．Tepwong P, Giri A, Sasaki F, et al.. Mycobial enhancement of ergothioneine by submerged cultivation ofedible mushroom mycelia and its application as an antioxidative compound[J]. Food Chemistry, 2012,131(1):247-258. [2]．Tabaraki R, Nateghi A. Optimization of ultrasonic-assisted extraction of natural antioxidants from rice bran using response surface methodology[J]. Ultrasonics Sonochemistry, 2011,18(6):1279-1286. [3]．Tabaraki R, Heidarizadi E, Benvidi A. Optimization of ultrasonic-assisted extraction of pomegranate (Punica granatum L.) peel antioxidants by response surface methodology[J]. Separation and Purification Technology, 2012,98:16-23. [4]．Setyaningsih W, Saputro I E, Palma M, et al.. Optimisation and validation of the microwave-assisted extraction of phenolic compounds from rice grains[J]. Food Chemistry, 2015,169:141-149. [5]．Repine J E, Elkins N D. Effect of ergothioneine on acute lung injury and inflammation in cytokine insufflated rats[J]. Preventive Medicine, 2012,54:S79-S82. [6]．Chen S, Ho K, Hsieh Y, et al.. Contents of lovastatin, γ-aminobutyric acid and ergothioneine in mushroom fruiting bodies and mycelia[J]. LWT-Food Science and Technology, 2012,47(2):274-278. [7]．Dubost N J, Beelman R B, Perterson D, et al.. Identification and Quantification of Ergothioneine in Cultivated Mushrooms by Liquid Chromatography-Mass Spectroscopy[J]. Internationnal Journal of Medicinal Mushroons, 2005,8(8):215-222. [8]．Encarnacion A B, Fagutao F, Hirayama J, et al.. Edible Mushroom (Flammulina velutipes) Extract Inhibits Melanosis in Kuruma Shrimp (Marsupenaeus japonicus)[J]. Journal of Food Science,2011,76(1):C52-C58. [9]．Encarnacion A B, Fagutao F, Hirono I, et al.. Effects of Ergothioneine from Mushrooms (Flammulina velutipes) on Melanosis and Lipid Oxidation of Kuruma Shrimp (Marsupenaeus japonicus)[J]. Journal of Agricultural and Food Chemistry, 2010,58(4):2577-2585. [10]．Ey J, Schouml;mig E, Taubert D. Dietary Sources and Antioxidant Effects of Ergothioneine[J]. Journal of Agricultural and Food Chemistry, 2007,55(16):6466-6474. [11]．Gr#252;ndemann D. The ergothioneine transporter controls and indicates ergothioneine activity -A review[J]. Preventive Medicine, 2012,54:S71-S74. [12]．Lee W Y, Park E, Ahn J K. Supplementation of Methionine Enhanced the Ergothioneine Accumulation in the Ganoderma neo-japonicum Mycelia[J]. Applied Biochemistry and Biotechnology, 2009,158(1):213-221.

3. 毕业设计（论文）进程安排

起讫日期 设计（论文）各阶段工作内容 2.10-3.1 确定毕业设计的课题及相关资料的查找 3.2-3.10 实验方案的设计 3.10-3.20 实验材料的准备 3.21-5.15 准备发酵实验 5.15-6.5 实验数据处理与结果分析 5.20-6.10 毕业论文书写