1. 毕业设计（论文）的内容和要求

1．文献查阅 掌握文献查阅的一般方法，学会使用计算机在中国期刊网、维普数据库、超星数字图书馆等中文数据库、ca、sci等外文数据库检索资源上查阅有关文献。

2．文献阅读及综述 阅读与课题相关的外文及中文文献，了解酶表达验证的方法及过程，撰写文献综述。

3．明确实验任务，拟定实验方案 根据所查阅文献的内容，确定实验内容及方案，拟定科学可行的研究计划。

2. 参考文献

1.Cloning and functional analysis of HpFAD2 and HpFAD3 genes encoding Δ12- and Δ15-fatty acid desaturases in Hansenula polymorpha 2.Analysis of #1113151;12-fatty acid desaturase function revealed that two distinct pathways are active for the synthesis of PUFAs in T. aureum ATCC 34304 3.Identification and characterization of Δ12 and Δ12/Δ15 bifunctional fatty acid desaturases in the oleaginous yeast Lipomyces starkeyi 4.Identification of a Δ12 fatty acid desaturase from oil palm (Elaeis guineensis Jacq.) involved in the biosynthesis of linoleic acid by heterologous expression in Saccharomyces cerevisiae 5.Biosynthetic mechanism of very long chain polyunsaturated fatty acids in Thraustochytrium sp. 2618 6.Functional characterization and substrate specificity analysis of D6-desaturase from marine microalga Isochrysis sp. 7.Fatty acid desaturase 3 (PsFAD3) from Paeonia suffruticosa reveals high α- T linolenic acid accumulation 8.Cloning and Functional Identification of Delta5 Fatty Acid Desaturase Gene and Its 5′-Upstream Region from Marine Fungus Thraustochytrium sp. FJN-10 9.Polyunsaturated fatty acids in marine bacteria and strategies to enhance their production 10.Characterization of Both Polyunsaturated Fatty Acid Biosynthetic Pathways in Schizochytrium sp.

3. 毕业设计（论文）进程安排

2.10-3.1 确定毕业设计的课题及相关资料的查找 3.2-3.10 实验方案设计 3.11-3.20 实验材料准备 3.21-5.15 分子实验 5.16-5.20 实验结果处理与分析 5.21-6.10 毕业论文书写