1. 毕业设计（论文）的内容和要求

1、独立完成开题报告及文献综述 2、熟练掌握实验技能，了解课题的相关知识 3、做好实验规划，及时完成进度要求 4、完成毕业论文，准备好开题答辩

2. 参考文献

Ledesma-Amaro, R., Nicaud, J.M., 2016. Metabolic engineering for expanding the substrate range of Yarrowia lipolytica. Trends Biotechnol. pii: S0167-7799(16) 30029-4. Madzak, C., Gaillardin, C., Beckerich, J.M., 2004. Heterologous protein expression and secretion in the non-conventional yeast Yarrowia lipolytica: a review. J. Biotechnol. 109, 63#8211;81. Nicaud, J.M., Madzak, C., van den Broek, P., Gysler, C., Duboc, P., Niederberger, P., Gaillardin, C., 2002. Protein expression and secretion in the yeast Yarrowia lipolytica. FEMS Yeast Res. 2, 371#8211;379. Ruiz-Herrera, J., Sentandreu, R., 2002. Different effectors of dimorphism in Yarrowia lipolytica. Arch. Microbiol. 178, 477#8211;483. Sanchez Nogue, V., Karhumaa, K., 2015. Xylose fermentation as a challenge for commercialization of lignocellulosic fuels and chemicals. Biotechnol. Lett. 37, 761#8211;772. Lazar, Z., Walczak, E., Robak, M., 2011. Simultaneous production of citric acid and invertase by Yarrowia lipolytica SUCt transformants. Bioresour. Technol. 102, 6982#8211;6989. Xue Z, Sharpe PL, Hong SP, Yadav NS, Xie D, Short DR, Damude HG, Rupert RA, Seip JE, Wang J, et al. Production of omega-3 eicosapentaenoic acid by metabolic engineering of Yarrowia lipolytica. Nat Biotechnol. 201 Tai M, Stephanopoulos G. Engineering the push and pull of lipid biosynthesis in oleaginous yeast Yarrowia lipolytica for biofuel production. Metab Eng. 2013;15:1#8211;9

3. 毕业设计（论文）进程安排

1、了解课题，完成文献翻译和开题报告 2、成功构建能够高效利用木糖的解脂耶氏酵母 3、探索重组菌的生长状况及发酵性能 4、了解重组菌的作用及应用前景