1. 毕业设计（论文）的内容和要求

首先写产琥珀酸放线杆菌的综述，包括底物利用，产酸能力，代谢途径介绍；其次实验方法，实验所需试剂以及设备；最后对实验结果进行分析。

2. 参考文献

Okino S, Noburyu R, Suda M, et al. An efficient succinic acid production process in a metabolically engineered Corynebacterium glutamicum strain[J]. Applied Microbiology and Biotechnology, 2008, 81(3): 459-464. Blankschien M D, Clomburg J M, Gonzalez R. Metabolic engineering of Escherichia coli for the production of succinate from glycerol[J]. Metabolic Engineering, 2010, 12(5): 409-419. Lin H, Bennett G N, San K-Y. Chemostat culture characterization of Escherichia coli mutant strains metabolically engineered for aerobic succinate production: a study of the modified metabolic network based on metabolite profile, enzyme activity, and gene expression profile[J]. Metabolic Engineering, 2005, 7(5): 337-352.

3. 毕业设计（论文）进程安排

看文献，了解试验菌株 实验设计 进行试验 实验结果分析