1. 毕业设计（论文）的内容和要求

1、采用计算机检索20篇中文文献和10篇英文文献； 2、阅读20篇文献，翻译一篇英文文献，提交一篇开题报告。

格式要求为：参考文献标注符合规范，计算机打印； 3、实验工作主要内容本课题以纤维二糖差向异构酶为研究对象，以提高乳果糖的产量和降低成本为目的，进行系统的酶法转化生产乳果糖工艺的设计和优化研究。

通过测定酶活以及转化率，分别对诱导条件以及催化制备乳果糖的转化条件进行研究，为工业化生产乳果糖奠定基础。

2. 参考文献

[1]李凤林，李明阳.乳果糖的制备方法与功能研究进展[J].饮料工业,2012,15:9#8211;12 [2]杨思行，吴昊，郑小平，等.乳酮糖及其在食品上的应用[J].食品与机械,1998,6：25#8211;26 [3]于海，杜昱光，韩秀文.乳果糖的制备分离及纯化的研究发展现状.中国微生态学杂志,1998,10(3)：184#8211;186 [4] Ruttloff H, Taufel A, Krause W, Haenel H, Taufel K. Enzymatic hydrolysis of galactooligosaccharides in the human and animal intestine with particular regard to L.bifidus[J]. Behavior of lactulose in the intestine ,1967,11:39#8211;46. [5] Wollowski I, Rechkammer G, Pool-Zobel BL. Protective role of probiotics and prebiotics in colon cancer. Am J Clinical Nutr ,2001,73:451#8211;5 [6] Tabatabaie T, Kotake Y, Wallis G, Jacob JM, Floyd RA. Spin trapping agent phenyl-Ntertbutylnitrone protects against the onset of drug-induced insulin-dependent diabetes mellitus. FEBS Letts,1997,407:148#8211;52. [7] Schumann C. Die immunologischen Effekte der Lactulose. Notabene medici 1997;27:288#8211;90. [8] Playne MJ, Crittenden RG. Galacto-oligosaccharide and other products derived from lactose. Advanced dairy chemistry: lactose,water, salts and major constituents, 2009,3: 155. [9] Parmjit S. Panesar ,Shweta Kumari. Lactulose: Production, purification and potential applications[J]. Biotechnology Advances, 2011,29 :940#8211;948 [10]Seyyedhadi K,Farzin Z A,Babak B,et al. The enzymatic production of lactulose via transglycosylation in conventional and non-conventional media[J]. International Dairy Journal，2014，34(1): 74-79 [11]Hua X,Yang R,Zhang W,et al. Dual-enzymatic synthesis of lactulose in organic-aqueous two-phase media[J]. Food Research International，2012，43(3): 716-722 [12]Panesr P S,Kumari S. Lactulose: production, purification and potential applications[J]. Biotechnology Advances，2011，29(6): 940-948 [13]Kim Y S,Oh D K. Lactulose production from lactose as a single substrate by a thermostable cellobiose 2-epimerase from Caldicellulosiruptor saccharolyticus[J]. Bioresource Technology，2012，104: 668-672 [14]Tyler T,Leatherwood J. Epimerization of disaccharides by enzyme preparations from Ruminococcus albus[J]. Archives of Biochemistry and Biophysics，1967，119: 363-367 [15]顾俊艳.纤维二糖差向异构酶的分离纯化及固定化研究[D].无锡：江南大学，2014 [16]Kim J E,Kim Y S,Kang L W,et al. Characterization of a recombinant cellobiose 2-epimerase from Dictyoglomus turgidum that epimerizes and isomerizes β-1,4-and α-1,4-gluco-oligosaccharides[J]. Biotechnology letters，2012，34(11): 2061-2068 [17] Park C S,Kim J E,Choi J G,et al. Characterization of a recombinant cellobiose 2-epimerase from Caldicellulosiruptor saccharolyticus and its application in the production of mannose from glucose[J].Applied Microbiology and Biotechnology,2011,92(6): 1187一1196 [18]杨瑞金，汪明明，华霄，张文斌，沈秋云. 一种全细胞催化产乳果糖的方法[P].中国：201410262301.1，2014-08-27 [19]Wang M M, Yang R J, Hua X, Shen Q Y, Zhang W B, Wei Zhao. Lactulose production from lactose by recombinant cellobiose 2-epimerase in permeabilised Escherichia coli cells[J]. International Journal of Food Science and Technology, 2015, 50, 1625#8211;1631

3. 毕业设计（论文）进程安排

起讫日期 设计（论文）各阶段工作内容

2015.10.8#8212;2015.11.6 确定论文题目、填写任务书

2015.11.7--2015.12.6 查找资料、写开题报告