1. 毕业设计（论文）的内容和要求

（1）掌握文献查阅的一般方法，学会在中国期刊网、web of science科学引文索引、springer link电子期刊等检索资源上查阅关于酿酒酵母全细胞催化合成莱鲍迪甙a（ra甙）研究的相关文献，并对重组酿酒酵母菌对外源基因的表达和调控方式有全面了解。

（2）文献阅读及综述：阅读与课题相关的中英文文献，了解国内外的研究动态，撰写文献综述。

（3）明确实验任务，拟定实验方案：根据所查阅文献的内容，确定研究内容及方案，拟定科学可行的研究计划。

2. 参考文献

[1] Alex Richman, Andrew Swanson, Tania Humphrey, et al. Functional genomics uncovers three glucosyltransferases involved in the synthesis of the major sweet glucosides of Stevia rebaudiana[J]. The Plant Journal,2005,41(1):56-67. [2] de Carvalho, C. C. C. R. Enzymatic and whole cell catalysis: Finding new strategies for old processes. Biotechnology Advances, 2001, 29(1):, 75-83. [3] 刘欢,李艳,严明等.甜叶菊糖基转移酶UGT76G1的克隆表达及其性质研究[J].食品工业科技,2012,33(20): 187-190. [4] 刘欢,李艳,严明等.重组酿酒酵母全细胞催化合成莱鲍迪苷A[J].食品与发酵工业, 2012, 38( 7): 6-10. [5] 王钰,陈量量,杜婷,李艳等.酿酒酵母组成型表达甜叶菊糖基转移酶UGT76G1及相关性质研究[J].食品工业科学. 2015:162-165. [6] De Bruyn, F., Maertens, J., Beauprez, J., et al Soetaert, W., De Mey, M. Biotechnological advances in UDP-sugar based glycosylation of small molecules [J]. Biotechnology Advances, 2015, 33(2):288-302. [7] Ruffing A, Chen RR. Metabolic engineering of microbes for oligosaccharide and polysaccharide synthesis[J]. Microbial Cell Factories, 2006, 5(1):25. [8] Mao Z, Shin HD, Chen RR. Engineering the E. coli UDP-glucose synthesis Pathway for oligosaccharide Synthesis[J].Biotechnology Progress,2006,22(2):369#8722;374. [9]Yan Y,Li Z,Mattheos AGK. High-Yield anthocyanin biosynthesis in engineered Escherichia coli[J]. Biotechnology and Bioengineering, 2008,100(1):126-140. [10] Munch-Petersen A, Kalckar H M, Cuyolo E,Smith E E, et al. Uridyl transferases and the formation of uridine triphosphate[J].Nature,1953, 172:1036#8211;1037. [11] Daran JM, Dallies N, Thinessempoux D, Paquet V, Francois J, et al. Genetic and biochemical characterization of the UGP1 gene encoding the UDP-glucose pyrophosphorylase from Saccharomyces cerevisiae[J]. Biochem, 1995,233: 520#8211;530. [12]Eimert K, Villand P, Kilian A, Kleczkowski LA, et al. Cloning and characterization of several cDNAs for UDP-glucose pyrophosphorylase from barley (Hordeum vulgare) tissues[J]. Gene 1996,170: 227#8211;232. [13]萨姆布鲁克 J,拉塞尔 DW.分子克隆实验指南［M］.黄培堂,译.3版.北京: 科学出版社,2002: 1601－1604.

3. 毕业设计（论文）进程安排

2016.1-2016.3 熟悉实验原理和实验操作，查阅文献，对课题进行初步探索。

2016.3-2016.4 考察重组酿酒酵母生长代谢状态。

2016.4-2016.5 研究酿酒酵母重组菌yph499 (pescd-ugt-ugpase）全细胞催化低品质甜菊生成莱鲍迪甙a。