论文总字数：21519字

摘 要

L－天冬氨酸(L－Aspartic acid)，在医药、食品、美容、化工、材料等方面具有非常广泛的用途。L-天冬氨酸常用于氨基酸输液及降压、平喘、消化性溃疡等，并可用于治疗心肌梗死、心力衰竭,疲劳症和肿瘤等,还可以合成的阿斯巴甜和纽甜。可见在不久的将来，L－天冬氨酸的运用会越来越广泛，需求也会越来越大。

目前L－天冬氨酸的制备主要是利用 L－天冬氨酸酶催化氨与富马酸发生加成反应得到的，L－天冬氨酸酶在 L-天冬氨酸工业生产中具有重要作用，想要提高L－天冬氨酸产量，就必须提高L－天冬氨酸酶的酶活和L－天冬氨酸酶产生菌的产量。而本次课题就是研究L－天冬氨酸酶的产酶条件如何优化。

关键词：L-天冬氨酸酶，大肠杆菌，条件优化，酶活

窗体顶端

窗体底端

Optimization of Enzyme Production Conditions of L - aspartate Producing

Abstract

L－Aspartic acid ，having been widely used in medicine , fodstuff , hairdressing , material and so on . L－Aspartic acid can using for acid infusion , depressurization , relieving asthma , peptic ulcer , and treating MI , heart failure , apocamnosis and tumour，and L－Aspartic acid is raw material of APM and neotame. In the future , L－Aspartic acid will be used widely and demand more and more .

L-aspartase plays an important role in L-aspartic acid production , it is necessary to increase the activity of L-aspartase and L-aspartase Production of acidase producing bacteria , if you want to increase L-aspartic acid production . And this issue is to study how to optimize L-aspartase enzyme production conditions .

Key word : L-aspartase； E.coli ；optimization ； Enzyme activity

窗体底端

目录

第一章 绪论·······································1

1.1 前言 ········································1

1.2 L-天冬氨酸简介····································1

1.3 L-天冬氨酸酶·····································1

1.4 L-天冬氨酸酶·····································2

第二章 实验准备·····································3

2.1 实验材料 ······································3

2.1.1 实验试剂······································3

2.1.2 实验仪器······································3

2.1.3 培养基·······································4

2.1.3.1 固体培养基····································4

2.1.3.2 液体培养基····································4

2.1.3.3 发酵培养液····································4

2.1.3.4 正交培养液····································5

2.2 实验方法 ······································5

2.2.1 实验流程······································5

2.2.2 大肠杆菌数量测定方法································7

2.2.3 酶活测定方法····································7

2.2.4 测量富马酸标准曲线及测量结果····························8

2.2.5 大肠杆菌种子的准备································10

2.2.6 底物配置·····································11

第三章 发酵产酶实验··································12

3.1 实验过程······································12

3.1.1 碳源对大肠杆菌产酶影响的实验···························12

3.1.2 ph对大肠杆菌产酶影响的实验····························12

3.1.3 接种量对大肠杆菌产酶影响的实验··························13

3.1.4 培养时间对大肠杆菌产酶影响的实验··························14

3.1.5 培养温度对大肠杆菌产酶影响的实验·························14

3.1.6正交实验过程····································15

3.1.7 验证性实验过程···································15

3.2 实验结果······································15

3.2.1 碳源对大肠杆菌产酶的影响·····························15

3.2.2 ph对大肠杆菌产酶的影响······························16

3.2.3 接种量对大肠杆菌产酶的影响····························17

3.2.4 培养时间对大肠杆菌产酶的影响···························18

3.2.5 培养温度对大肠杆菌产酶的影响···························18

3.2.6 正交实验结果···································19

3.2.7 验证性实验结果··································20

第四章 小结及展望···································21

4.1 实验结论······································21

4.2 未来展望······································21

参考文献········································22

谢辞··········································23

剩余内容已隐藏，请支付后下载全文，论文总字数：21519字