1. 毕业设计（论文）的内容和要求

利用特定的cu2 促进罗丹明开环反应，开发出一个兼具水溶性和细胞渗透性的荧光淬灭型探针。

并通过1h-nmr、ms等分析方法对其结构进行表征，为合成多通路cu2 上转换纳米做前期准备。

当探针和cu2 作用时，结构由红色荧光且共轭结构转化为无荧光的非共轭结构，因此检测体系发生光转换。

2. 参考文献

(1) Vulpe, C.; Levinson, B.; Whitney, S.; Packman, S.; Gitschier, J. ISOLATION OF A CANDIDATE GENE FOR MENKES DISEASE AND EVIDENCE THAT IT ENCODES A COPPER-TRANSPORTING ATPASE. Nat. Genet. 1993, 3 (1), 7-13. (2) Zhang, L.; Lichtmannegger, J.; Summer, K. H.; Webb, S.; Pickering, I. J.; George, G. N. Tracing Copper-Thiomolybdate Complexes in a Prospective Treatment for Wilson's Disease. Biochemistry 2009, 48 (5), 891-897. (3) Wang, F.; Liu, X. Upconversion multicolor fine-tuning: Visible to near-infrared emission from lanthanide-doped NaYF4 nanoparticles. J. Am. Chem. Soc. 2008, 130 (17), 5642- . (4) Wang, F.; Xue, X.; Liu, X. Multicolor tuning of (Ln, P)-Doped YVO4 nanoparticles by single-wavelength excitation. Angew. Chem. Int. Ed. 2008, 47 (5), 906-909. (5) Zhou, J.; Liu, Z.; Li, F. Upconversion nanophosphors for small-animal imaging. Chem. Soc. Rev. 2012, 41 (3), 1323-1349. (6) Haase, M.; Schaefer, H. Upconverting Nanoparticles. Angew. Chem. Int. Ed. 2011, 50 (26), 5808-5829. (7) Yu, M.; Li, F.; Chen, Z.; Hu, H.; Zhan, C.; Yang, H.; Huang, C. Laser Scanning Up-Conversion Luminescence Microscopy for Imaging Cells Labeled with Rare-Earth Nanophosphors. Anal. Chem. 2009, 81 (3), 930-935. (8) Xiong, L.; Chen, Z.; Tian, Q.; Cao, T.; Xu, C.; Li, F. High Contrast Upconversion Luminescence Targeted Imaging in Vivo Using Peptide-Labeled Nanophosphors. Anal. Chem. 2009, 81 (21), 8687-8694. (9) Wang, F.; Han, Y.; Lim, C. S.; Lu, Y.; Wang, J.; Xu, J.; Chen, H.; Zhang, C.; Hong, M.; Liu, X. Simultaneous phase and size control of upconversion nanocrystals through lanthanide doping. Nature 2010, 463 (7284), 1061-1065. (10) Zhou, J.; Sun, Y.; Du, X.; Xiong, L.; Hu, H.; Li, F. Dual-modality in vivo imaging using rare-earth nanocrystals with near-infrared to near-infrared (NIR-to-NIR) upconversion luminescence and magnetic resonance properties. Biomaterials 2010, 31 (12), 3287-3295. (11) Idris, N. M.; Li, Z.; Ye, L.; Sim, E. K. W.; Mahendran, R.; Ho, P. C.-L.; Zhang, Y. Tracking transplanted cells in live animal using upconversion fluorescent nanoparticles. Biomaterials 2009, 30 (28), 5104-5113. (12) Chen, Z.; Chen, H.; Hu, H.; Yu, M.; Li, F.; Zhang, Q.; Zhou, Z.; Yi, T.; Huang, C. Versatile synthesis strategy for carboxylic acid-functionalized upconverting nanophosphors as biological labels. J. Am. Chem. Soc. 2008, 130 (10), 3023-3029. (13) Zijlmans, H.; Bonnet, J.; Burton, J.; Kardos, K.; Vail, T.; Niedbala, R. S.; Tanke, H. J. Detection of cell and tissue surface antigens using up-converting phosphors: A new reporter technology. Anal. Biochem. 1999, 267 (1), 30-36.

3. 毕业设计（论文）进程安排

2018.1.2-2018.3.1 查阅资料，完成开题报告和任务书 2018.3.2-2018.3.10 确定方案，学习实验过程和操作 2018.3.11-2018.4.30 独立实验，合成产品并进行结构表征 2018.5.1-2018.5.30 数据整理，书写论文，制作PPT 2018.5.30- 修改论文并完成答辩