1. 毕业设计（论文）的内容和要求

益生菌是指能以一定数量存活并定殖于宿主肠道内，通过调节肠道菌群平衡对宿主健康发挥有益作用的单一或者特定微生物混合物[1]。

主要的益生菌分为两类：双歧杆菌和乳杆菌。

而脂肪酸在日常的生活中得到广泛应用，短链脂肪酸能维持身体的机能，膳食中的总饱和脂肪酸含量与人体血清中高密度脂蛋白胆固醇（hdl-c）呈正相关,增加膳食中总脂和饱和脂肪的含量不仅会增加ldl-c含量,也会增加hdl-c的含量[2]。

2. 参考文献

[1] Ho, S. Y.; Chen, F. Lipid characterization of Mortierella alpine grown at different NaCl concentrations. J. Agric. Food Chem. 2008, 56, 7903#8722;7909. [2] Ji, X. J.; Ren, L. J.; Nie, Z. K.; Huang, H.; Ouyang, P. K. Fungal arachidonic acid-rich oil: Research, development, and industrialization. Crit. Rev. Biotechnol. 2014, 34, 197#8722;214. [3] Sui, X.; Niu, X.; Shi, M.; Pei, G.; Li, J.; Chen, L.; Wang, J.; Zhang, W. Metabolomic analysis reveals mechanism of antioxidant butylated hydroxyanisole on lipid accumulation in Crypthecodinium cohnii. J. Agric. Food Chem. 2014, 62, 12477#8722;12484. [4] Dyal, S. D.; Narine, S. S. Implications for the use of Mortierella fungi in the industrial production of essential fatty acids. Food Res. Int. 2005, 38, 445#8722;467. [5] Ho, S. Y.; Jiang, Y.; Chen, F. Polyunsaturated fatty acids (PUFAs) content of the fungus Mortierella alpina isolated from soil. J. Agric. Food Chem. 2007, 55, 3960#8722;3966. [6] Jin, M. J.; Huang, H.; Xiao, A. H.; Zhang, K.; Liu, X.; Li, S.; Peng, C. A novel two-step fermentation process for improved arachidonic acid production by Mortierella alpina. Biotechnol. Lett. 2008, 30, 1087#8722;1091. [7] Li, X.; Ye, Y.; Chang, M.; Jin, Q.; Wang, X. Efficient production of arachidonic acid by Mortierella alpina through integrating fed-batch culture with a two-stage pH control strategy. Bioresour. Technol. 2015, 181, 275#8722;282. [8] Peng, C.; Huang, H.; Ji, X. J.; Liu, X.; You, J. Y.; Lu, J. M.; Cong, L. L.; Xu, X. K.; Ouyang, P. K. A temperature-shift strategy for efficient arachidonic acid fermentation by Mortierella alpina in batch culture. Biochem. Eng. J. 2010, 53,92#8722;96. [9] Ji, X. J.; Zhang, A. H.; Nie, Z. K.; Wu, W. J.; Ren, L. J.; Huang, H. Efficient arachidonic acid-rich oil production by Mortierella alpine through a repeated fed-batch fermentation strategy. Bioresour. Technol. 2014, 170, 356#8722;360. [10] Nie, Z. K.; Deng, Z. T.; Zhang, A. H.; Ji, X. J.; Huang, H. Efficient arachidonic acid-rich oil production by Mortierella alpine through a three-stage fermentation strategy. Bioprocess Biosyst. Eng. 2014, 37, 505#8722;511. [11] Jin, M. J.; Huang, H.; Xiao, A. H.; Gao, Z.; Liu, X.; Peng, C. Enhancing arachidonic acid production by Mortierella alpina ME-1 using improved mycelium aging technology. Bioprocess Biosyst. Eng. 2009, 32, 117#8722;122. [12] Osiewacz, H. D. Aging and longevity in the #64257;lamentous fungus Podospora anserina.In Aging of organisms: Biology of aging and its modulation; Osiewacz, H. D., Ed.; Springer: Netherlands, 2003; Vol. 4, pp 31#8722;53. [13] Ouyang, L. L.; Chen, S. H.; Li, Y.; Zhou, Z. G. Transcriptome analysis reveals unique C4-like photosynthesis and oil body formation in an arachidonic acid-rich microalga Myrmecia incise Reisigl H4301.BMC Genomics 2013, 14, 396. [14] Ratledge, C. The role of malic enzyme as the provider of NADPH in oleaginous microorganisms: a reappraisal and unsolved problems. Biotechnol. Lett. 2014, 36, 1557#8722;1568.

3. 毕业设计（论文）进程安排

起讫日期 设计（论文）各阶段工作内容 2017.12.1-12.5 与导师会面，布置论文题目及要求 12.6-12.22 查阅资料，完成开题报告和任务书 12.22-2018.1.12 完成翻译，检索相关文献 1.12-3.1 测试生长情况 3.2-4.30 特性测试 4.31-5.31 数据整理，书写论文,制作PPT 6.1-6.10 准备论文答辩