论文总字数：18819字

摘 要

酶交联的水凝胶具有亲水性和聚三维网络。因为水凝胶独特的性质，如高含水量、很不错的生物的相容特性、低炎症反应和与真正的组织相似的机械性能，现在水凝胶已经在医学方面会得到了各种应用，如药物的控制释放、生物的传感工程和组织工程。这几年来在原位形成的水凝胶或注射水凝胶已经得到越来越高的评价。由于原位形成水凝胶的生物分子的活性和细胞均匀能力，使他们能通过注射植入实现一个简单混合。更重要的是水凝胶具有可以填充任何形状的优势，因此在临床的方面，原位形成水凝胶在许多生物医学应用方面更为理想。

在酶交联的苯酚聚合物的存在下，通过辣根过氧化物酶(HRP)和过氧化氢（H₂O₂）来合成γ-聚谷氨酸(γ-PGA-DA) 水凝胶。研究γ-聚谷氨酸水凝胶溶胀、降解以及止血等方面的特性。

实验表明γ- PGA-DA的浓度、H₂O₂的浓度和HRP的浓度都影响着γ-聚谷氨酸水凝胶的溶胀行为、凝胶时间以及降解性能。此外γ-聚谷氨酸水凝胶在止血领域有着显著的功效。γ-聚谷氨酸水凝胶有望在药物的控制释放和组织工程领域中成为一种优良的原材料。

关键词：γ-聚谷氨酸 水凝胶 酶交联

Preparation and application of a kind of gamma poly glutamic acid hydrogel

Abstract

The medical wound dressing hydrogel is a new type of wound dressing developed rapidly in recent years. Compared with conventional hydrogel wound dressing can promote wound healing better, reduce the pain of the wound, he can be used to improve the surface micro environment of the trauma, inhibited the growth of bacteria. The hydrogel is very suitable for common body trauma such as, abrasions, scratches and other skin trauma. For these wounds, common cases doctors will use sterile gauze and topical antibiotics for treatment. But the gauze easily with the skin wound tissue adhesions, change medicine often disrupts epithelial and granulation tissue of newborn, causing bleeding. This will not only not conducive to wound healing, and the wound will make the patient more pain. Water gel dressing on the wound, he is not going to stick with the wound tissue, without destroying the new organization , but also can kill a variety of bacteria, to avoid the infection of the wound.

Acrylic acid and its derivatives are common in synthetic hydrogels, monomer acrylamide derivatives and other synthetic hydrogel has good stability, but its biodegradable and biocompatible to poor. The main raw material for a natural class of hydrogels of chitosan, sodium alginate, cellulose, starch, etc.. Because of these natural polysaccharide has good biological compatibility and biodegradation of, but also it is inexpensive and can be, therefore, a natural class of hydrogels in the field of wound dressing and drug controlled release has more advantages.

Are reviewed in this paper in the presence of cross-linked enzyme phenol polymer, by horseradish peroxidase (HRP) and hydrogen peroxide (H₂O₂) to synthesize gamma polyglutamic acid (PGA) hydrogel. An overview of the gamma poly glutamic acid hydrogel for wound dressing, controlled drug release and tissue engineering application. At last, it discusses the gamma poly glutamic acid hydrogel of research and application.

Key words: Gamma poly glutamic acid;Hydrogel;Enzyme crosslinking

目录

摘要 I

Abstract II

目录 III

第一章 文 献 综 述 1

1.1 引言 1

1.2 γ-聚谷氨酸 2

1.2.1 γ-聚谷氨酸的吸水特性 2

1.2.2 γ-聚谷氨酸的生物可降解性 2

1.2.3 γ-聚谷氨酸的水解特性 3

1.2.4 γ-聚谷氨酸的止血特性 3

1.2.5 γ-聚谷氨酸可促进皮肤组织修复和伤口愈合 3

1.2.6 γ-聚谷氨酸的安全性 3

1.2.7 γ-聚谷氨酸应用于水凝胶的特点与优势 3

1.3 γ-聚谷氨酸水凝胶 4

1.3.1 γ-聚谷氨酸水凝胶的研究现状 4

1.3.2 γ-聚谷氨酸水凝胶的应用 4

1.3.2.1组织工程材料 4

1.3.2.2药物载体材料 4

1.3.2.3 创面修复材料 5

1.3.2.4环境领域 5

1.3.2.5食品领域 6

1.4 研究的意义和目的 6

第二章 实验部分 7

2.1实验材料 7

2.1.1实验试剂 7

2.1.2实验设备 7

2.2 γ-聚谷氨酸-多巴胺（γ-PGA-DA）的合成 8

2.3 γ-聚谷氨酸-多巴胺（γ-PGA-DA）的表征 8

2.3.1 氢谱核磁共振（HNMR） 8

2.3.2 紫外可见分光光度计表征 9

2.3.3 凝胶时间测量 9

2.3.4 水凝胶形貌表征 9

2.3.5 水凝胶溶胀性能 9

2.3.6 水凝胶降解性能 9

2.3.7 伤口止血实验 10

2.4结果与讨论 10

2.4.1 γ-PGA-DA水凝胶的合成 10

2.4.2 γ-PGA-DA水凝胶的表征 10

2.4.2.1 氢谱核磁共振（HNMR） 10

2.4.2.2 紫外可见分光光度计表征 11

2.4.2.3 γ-PGA-DA水凝胶的配制 12

2.4.2.4 凝胶时间测量 12

2.4.2.5 水凝胶微观形貌表征 14

2.4.2.6 水凝胶溶胀性能 14

2.4.2.7 水凝胶降解性能 15

2.4.2.8 动物止血实验 16

2.5 结论与展望 16

参考文献 18

致谢 20

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