1. 毕业设计（论文）的内容和要求

海藻酸钙(calculate-sodium alginate, ca-sa)基胶囊是能够将活性物质等芯材包覆于囊芯的优良固定化体系，具有较大的空腔，能为囊芯提供固定化空间，同时囊膜对囊芯具有一定的阻隔作用，可减少芯层物质的泄漏、延缓药释放等，已广泛应用于生物化工、药妆、食品等各领域。

目前，ca-sa基胶囊存在的主要问题之一是，非水环境下储存时，胶囊极易失水塌陷，缩短了胶囊的使用寿命。

本课题拟通过静电纺丝法改进ca-sa基胶囊的制备工艺，确定胶囊的最佳制备条件，并通过壳聚糖(chitosan, cs)对胶囊囊壁改性，降低胶囊失重率，延长其使用期。

2. 参考文献

[1] Messner M, Kurkov S V, Jansook P, et al. Self-assembled cyclodextrin aggregates and nanoparticles[J]. International Journal of Pharmaceutics, 2010, 387: 199-208. [2] Lee Hyun-Ho, Park Oh-Jin. Continuous Production of Uniform Calcium Alginate Beads by Sound Wave Induced Vibration[J]. Journal of Chemical Technology Biotechnology, 1996, 67: 255-259. [3] Kofuji K, Isobe T, Murata Y. Controlled release of alpha-lipoic acid through incorporation into natural polysaccharide-based gel beads[J]. Food Chemistry, 2009, 115(2): 483-487. [4] Coppi G, Iannuccelli V. Alginate/chitosan microparticles for tamoxifen delivery to the lymphatic system[J]. Int. J. Pharm., 2009, 367(1-2): 127-132. [5] Kumar S, Dwevedi A, Kayastha A M. Immobilization of soybean (Glycine max) urease on alginate and chitosan beads showing improved stability: Analytical applications[J]. Journal of Molecular Catalysis B: Enzymatic, 2009, 58(1-4): 138-145. [6] Wittaya-areekul S, Kruenate J, Prahsarn C. Preparation and in vitro evaluation of mucoadhesive properties of alginate/chitosan microparticles containing prednisolone[J]. Int. J. Pharm., 2006, 312(1-2): 113-118. [7] Yapar E, Kayahan S K, Bozkurt A, et al. Immobilizing cholesterol oxidase in chitosan#8211;alginic acid network [J]. Carbohydrate Polymers, 2009, 76(3): 430-436. [8] Hwang Y S, Cho J, Tay F, et al. The use of murine embryonic stem cells, alginate encapsulation, and rotary microgravity bioreactor in bone tissue engineering [J]. Biomaterials, 2009, 30(4): 499-507. [9] Sakai S, Hashimoto I, Kawakami K. Development of alginate-agarose subsieve-size capsules for subsequent modification with a polyelectrolyte complex membrane[J]. Biochem. Eng., 2006, 30(1): 76-81. [10]林丹丹.电纺丝法制备ZnO纳米纤维及其性能研究[D].北京:清华大学,2009:45-47. [11]王莹熇,王进贤,董相廷,等.静电纺丝制备Eu(BA)3phen/PANI/PVP光电双功能复合纳米纤维及其性能[J].高等学校化学学报, 012,33:1657-1662. [12]刘袖洞,于炜婷,马小军,等.海藻酸钠和壳聚糖聚电解质微胶囊及其生物医学应用[J] .化学进展,2008,20 (1):126-139. [13]薛伟明,于炜婷,刘袖洞,等.载细胞海藻酸钠/壳聚糖微胶囊的化学破囊方法研究[J] .高等学校化学学报,2004,25(7):1342-1346. [14]付颖丽,雄鹰,刘袖洞,等. 海藻酸钠-壳聚糖微胶囊固定化大肠杆菌的研究[J].生物工程学报,2002,18(2):239-241. [15]温慧云,齐志涛,薛伟明,等.Fe3O4/海藻酸盐微胶囊固定化细胞生长特性[J].食品与发酵工业,2009,35(4):51-55.

3. 毕业设计（论文）进程安排

起讫日期 设计（论文）各阶段工作内容 11.28-12.02 与学生见面，布置任务，对论文说明 12.05-12.16 查阅资料 12.19-12.23 文献综述，撰写开题报告 12.26-12.30 实验准备工作 01.02-06.02 进行毕业论文相关实验 06.05-06.16 论文撰写、修改 06.19-06.23 论文撰写、修改，论文答辩