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毕业论文网 > 任务书 > 化学化工与生命科学类 > 轻化工程 > 正文

线粒体双光子荧光示踪剂的设计、合成与生物成像任务书

 2020-05-10 02:42:22  

1. 毕业设计(论文)的内容和要求

毕业论文是对大学四年级上学期学习情况以及对所做的课题情况的反映,其内容应该包括:

a) 查阅相关文献资料,提出实验计划,明确实验内容。

b) 了解课题的研究目的,进行大量的实验,了解线粒体荧光探针的应用状况和制备方法。掌握有机合成荧光探针的制备工艺,并分析讨论单光子和双光子荧光探针对线粒体成像的影响,并在此基础上总结实验经验及注意事项,按要求完成毕业论文的写作。

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2. 参考文献

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[2] ZHANG C, LIU T, SU Y P, et al. A near-infrared fluorescent heptamethine indocyanine probe with preferential tumor accumulation for in vivo imaging [J]. Biomaterials, 2010, 31(25):6612-6617.
[3] HAN J, HAN M S, TUNG C. A non-toxic fluorogenic probe for mitochondria labeling [J]. Biochimica et Biophysica Acta (BBA)-General Subjects, 2013, 1830(11): 5130-5135.
[4] KAWAZOE Y, SHIMOGAWA H, SATO A, et al. A mitochondrial surface-specific fluorescent probe activated by bioconversion [J]. Angewandte Chemie International Edition, 2011, 50(24): 5478-5481.
[5] MIAO F, ZHANG W J, SUN Y M, et al. Novel fluorescent probes for highly selective two-photon imaging of mitochondria in living cells [J]. Biosensors and Bioelectronics, 2014, 55: 423-429.
[6] DIVYA V, SANKAR V, RAGHU K G, et al. A mitochondriaspecific visible-light sensitized europium β-diketonate complex with
red emission [J]. Dalton Transactions, 2013, 42(34): 12317-12323.
[7] SENSI S L, TON-THAT D, WEISS J H, et al. A new mitochondrial fluorescent zinc sensor [J]. Cell Calcium, 2003, 34(3): 281-284.
[8] SREENATH K, ALLEN J R, DAVIDSON M W, et al. A FRET-based indicator for imaging mitochondrial zinc ions [J]. Chemical Communications, 2011, 47: 11730-11732.
[9] YOUNGAacute;BAEK N, HOAacute;HEO C, SUAacute;LIM C, et al. A highly sensitive two-photon fluorescent probe for mitochondrial zinc ions in living tissue [J]. Chemical Communications, 2012, 48(38):4546-4548.
[10] CHEN W D, GONG W T, YE Z Q, et al. FRET-based ratiometric fluorescent probes for selective Fe3 sensing and their applications in mitochondria [J]. Dalton Transactions, 2013, 42(28): 10093-10096.

[11] SHVARTSMAN M, FIBACH E, CABANTCHIK Z. Transferrin-iron routing to the cytosol and mitochondria as studied by live and real-time fluorescence [J]. Biochem. J., 2010, 429: 185-193.

[12] SHVARTSMAN M, CABANTCHIK Z I. Intracellular iron trafficking: role of cytosolic ligands [J]. Biometals, 2012, 25(4):711-723.
[13] MA Y M, ABBATE V, HIDER R C. Iron-sensitive fluorescent probes: monitoring intracellular iron pools [J]. Metallomics, 2015,7(2): 212-222.
[14] CABANTCHIK Z I. Labile iron in cells and body fluids: physiology, pathology, and pharmacology [J]. Frontiers in Pharmacology, 2014,5: 45. doi: 10.3389/fphar. 2014. 00045.
[15] TAKI M, AKAOKA K, MITSUI K, et al. A mitochondria-targeted turn-on fluorescent probe based on a rhodol platform for the detection of copper (Ⅰ) [J]. Organic amp; Biomolecular Chemistry, 2014, 12(27): 4999-5005.
[16] YUSOP R M, UNCITI-BROCETA A, JOHANSSON E M V, et al.
Palladium-mediated intracellular chemistry [J]. Nature, 2011, 3(3):239-243.
[17] LI H L, FAN J L, PENG X J. Colourimetric and fluorescent probes
for the optical detection of palladium ions [J]. Chemical Society Reviews, 2013, 42(19): 7943-7962.
[18] ZHANG S L, FAN J L, ZHANG S Z, et al. Lighting up fluoride ions in cellular mitochondria using a highly selective and sensitive fluorescent probe [J]. Chemical Communications, 2014, 50(90): 14021-14024.
[19] ASHTON T D, JOLLIFFE K A, PFEFFER F M. Luminescent probes for the bioimaging of small anionic species in vitro and in vivo [J]. Chemical Society Reviews, 2015, 44: 4547-4595.
[20] CHEN X Q, TIAN X Z, SHIN I, et al. Fluorescent and luminescent probes for detection of reactive oxygen and nitrogen species [J]. Chemical Society Reviews, 2011, 40(9): 4783-4804.
[21] ALBERS A E, OKREGLAK V S, CHANG C J. A FRET-based approach to ratiometric fluorescence detection of hydrogen peroxide[J]. Journal of the American Chemical Society, 2006, 128(30):9640-9641.
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3. 毕业设计(论文)进程安排

起讫日期

设计(论文)各阶段工作内容

备 注

2016.3~2016.4

查阅线粒体荧光探针的性能及制备相关文献资料

2016.4~2016.6

通过有机合成过程制备双光子荧光线粒体探针,并进行光学性质的测试与研究。

2016.5

进行中期检查

2016.5~2016.6

采用凝胶成像,荧光仪,酶标仪等对不同条件下合成产物靶向线粒体的性能进行测试。

实验和测试同时交叉进行

2016.6

在实验基础上撰写毕业论文

2016.6

论文答辩

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