1. 毕业设计（论文）的内容和要求

1、毕业论文内容 生物表面活性素（surfactin）通常是含有七个氨基酸（glu#8211;leu#8211;d#8211;leu#8211;val#8211;asp#8211;d#8211;leu#8211;leu ）的七肽与碳的个数为13-15的β-脂肪酸组成。

它是芽孢杆菌产生的一种次级代谢产物，具有可降解，低毒的特点，在医药[1]、食品[2]、生物修复[3]、生物防治[4]等方面具有很大的潜在应用价值[5, 6]。

在surfactin结构中，leu、val都是支链氨基酸，β-脂肪大多数也为支链脂肪酸。

2. 参考文献

[1] Zhai, S., Q. Shi, and X. Chen, Effect of dietary antimicrobial peptides-surfactin supplementation on parameters of intestinal health indices of genetically improved farmed tilapia (gift, oreochromis niloticus)[J]. Acta Hydrobiologica Sinica, 2016. 40(4): p. 823-829. [2] Zhang, Y., et al., Anti-inflammatory Activity and Mechanism of Surfactin in Lipopolysaccharide-Activated Macrophages[J]. Inflammation, 2015. 38(2): p. 756-764. [3] de Almeida Couto, C.R., et al., Response of the bacterial community in oil-contaminated marine water to the addition of chemical and biological dispersants. Journal Of Environmental Management[J], 2016. 184: p. 473-479. [4] Jasim, B., J. Mathew, and E.K. Radhakrishnan, Identification of a novel endophytic Bacillus sp from Capsicum annuum with highly efficient and broad spectrum plant probiotic effect[J]. Journal Of Applied Microbiology, 2016. 121(4): p. 1079-1094. [5] 翟少伟, 李剑,史庆超, 抗菌脂肽Surfactin的抗菌活性及应用[J]. 动物营养学报, 2015(05): p. 1333-1340. [6] 任鹏举, 枯草芽孢杆菌OKB105产生的surfactin防治烟草花叶病毒病及其机理研究[J]. 中国生物防治学报, 2014(02): p. 216-221. [7] Singh B K, Shaner D L. Biosynthesis of branched chain amino acids: From test tube to field[J]. The Plant Cell, 1995, 7(7): 935. [8] 刘丽霞, Surfactin合成酶相关基因研究[D]. 2012, 南京农业大学. [9] Liu Q, Lin J, Wang W, et al. Production of surfactin isoforms by Bacillus subtilis BS-37 and its applicability to enhanced oil recovery under laboratory conditions[J]. Biochemical Engineering Journal, 2015, 93: 31-37. [10] Huo Y, Zhan Y, Wang Q, et al. Acetolactate synthase (AlsS) in Bacillus licheniformis WX-02: enzymatic properties and efficient functions for acetoin/butanediol and l-valine biosynthesis[J]. Bioprocess and biosystems engineering, 2017: 1-10.

3. 毕业设计（论文）进程安排

12.1~12.31 文献查阅及资料研读 1.1~1.17 确定实验方案及开题报告写作 1.18~2.28 重组质粒与重组菌的构建 3.1~4.30 ALsS蛋白的纯化与酶活性质的研究 5.1~5.31 实验结果分析