1. 毕业设计（论文）的内容和要求

1．明确实验任务，拟定实验方案 根据所查阅文献的内容，确定实验内容及方案，拟定科学可行的研究计划。

2．开展实验研究 掌握极端嗜酸铁氧化微生物富集物培养及驯化、相关理化参数测定等基本操作，设计实验方案，并按计划进行实验。

3．处理实验数据和产品检测 实验过程中进行科学的实验数据记录；结束后，对数据进行合理处理，并对实验现象及结果进行科学分析、归纳总结，提出改进方案和可行建议，得出相应的结论。

2. 参考文献

参考文献 [1] Quatrini, R., C. Appia-Ayme, Y. Denis, et al. Extending the models for iron and sulfur oxidation in the extreme acidophile Acidithiobacillus ferrooxidans [J]. BMC Genomics, 2009, 10: 394-413. [2] Castelle, C., M. Guiral, G. Malarte, et al. A new iron-oxidizing/O2-reducing supercomplex spanning both inner and outer membranes, isolated from the extreme acidophile Acidithiobacillus ferrooxidans [J]. Journal of biological chemistry, 2008, 283(38): 25803-11. [3] Yarzabal, A., C. Appia-Ayme, J. Ratouchniak, et al. Regulation of the expression of the Acidithiobacillus ferrooxidans rus operon encoding two cytochromes c, a cytochrome oxidase and rusticyanin [J]. Microbiology, 2004, 150(7): 2113-2123. [4] Carbajosa, S., M. Malki, R. Caillard, et al. Electrochemical growth of Acidithiobacillus ferrooxidans on a graphite electrode for obtaining a biocathode for direct electrocatalytic reduction of oxygen [J]. Biosensors and Bioelectronics, 2010, 26(2): 877-80. [5] Satoshi Nakasono, N.M., Hiroshi Saiki. Electrochemical cultivation of Acidithiobacillus ferrooxidans by potential control [J]. Bioelectrochemistry and Bioenergetics, 1997, 43: 61-66. [6] Liu, W., J. Lin, X. Pang, et al. Overexpression of rusticyanin in Acidithiobacillus ferrooxidans ATCC19859 increased Fe(II) oxidation activity [J]. Current Microbiology, 2011, 62(1): 320-4. [7] Ahmadi, A., M. Schaffie, Z. Manafi, et al. Electrochemical bioleaching of high grade chalcopyrite flotation concentrates in a stirred bioreactor [J]. Hydrometallurgy, 2010, 104(1): 99-105. [8] 叶茂友, 严苹方,孙水裕,韩大建,庄圣炜,郑莉,黄绍松. 铜离子浓度对嗜酸性氧化亚铁硫杆菌生长特性和生长动力学的影响 [J]. 中国有色金属学报, 2016, 26(10), 2238-2245. [9] 邓蓉,张小云,张梦雪,廖美霞,曾子宁. 嗜酸氧化亚铁硫杆菌培养及其影响因素 [J]. 湘潭大学自然科学学报, 2014, 36(4), 67-71. [10] 方芳,钟宏,江放明,罗忠银,孙晓伟,许凯扬. 嗜酸氧化亚铁硫杆菌的耐砷驯化与浸矿能力 [J]. 中南大学学报, 2013, 44(10), 3977-3983. [11] 邓明强,白静,白建峰,罗新云,周举,王景伟. 影响嗜酸氧化亚铁硫杆菌生长及生物浸出效率的研究进展 [J]. 湿法冶金, 2016, 35(3), 171-175. [12] 刘宏伟,戴艳霞,黄伟,尹华群,梁伊丽,申丽,刘学端. 嗜酸异养菌对自养菌Acidithiobacillus ferrooxidans金属离子抗性和生物浸出的影响 [J]. 微生物学通报, 2012, 39(8), 1069#61485;1078. [13] 董颖博,林海,傅开彬,莫晓兰,温洪伟. 浮选药剂对嗜酸氧化亚铁硫杆菌浸出低品位铜尾矿的影响 [J]. 中国有色金属学报, 2011, 21(9), 2291-2297. [14] Van Khanh Nguyen, Mu Hyun Lee, Hyung Jun Park, Jong-Un Lee. Bioleaching of arsenic and heavy metals from mine tailings by pure and mixed cultures of Acidithiobacillus spp. [J]. Journal of Industrial and Engineering Chemistry, 2015, 21(25), 451-458. [15] LindaDekker, FlorenceArs#232;ne-Ploetze, Joanne M.Santini. Comparative proteomics of Acidithiobacillus ferrooxidans grown in the presence and absence of uranium [J]. Research in Microbiology, 2016, 167(3), 234-239. [16] Crist#243;bal Mart#237;nez-Bussenius, Claudio A.Navarroa Luis Orellana, Carlos A.Jerez. Global response of Acidithiobacillus ferrooxidans ATCC 53993 to high concentrations of copper: A quantitative proteomics approach [J]. Journal of Proteomics, 2016, 145(11), 37-45. [17] G.Cabrera, J.M.G#243;mez, D.Cantero. Kinetic study of ferrous sulphate oxidation of Acidithiobacillus ferrooxidans in the presence of heavy metal ions [J]. Enzyme and Microbial Technology, 2005, 36(2-3), 301-306. [18] G.Cabrera, J.M.G#243;mez, D.Cantero. Influence of heavy metals on growth and ferrous sulphate oxidation by Acidithiobacillus ferrooxidans in pure and mixed cultures [J]. Process Biochemistry, 2005, 40(8), 2683-2687. [19] Hee-Chan Jang, MarjorieValix. Overcoming the bacteriostatic effects of heavy metals on Acidithiobacillus thiooxidans for direct bioleaching of saprolitic Ni laterite ores [J]. Hydrometallurgy, 2017, 168, 21-25.

3. 毕业设计（论文）进程安排

毕业设计（论文）进程安排： 2017.12.22-2018.01.12 确定毕业设计的课题，查阅文献 2018.01.13 -2018.02.20 完善课题研究方案，完成外文翻译，文献综述 2018.02.21 -2018.03.21 实验材料的准备，菌种活化 2018.03.21 -2018.04.21 实验开展及数据记录 2018.04.21 -2018.06.01实验数据处理、结果分析及毕业论文撰写