1. 毕业设计（论文）的内容和要求

1 文献阅读与归纳，深入了解课题背景 2 大肠杆菌重组菌的诱导与表达 3 酶的纯化与多酶催化条件的优化 4 独立完成毕业论文的撰写

2. 参考文献

1 He Y, Li N, Chen Y, et al. Cloning, expression, and characterization of an adenylate cyclase from Arthrobacter sp. CGMCC 3584.[J]. Applied Microbiology Biotechnology, 2012, 96(4):963-970. 2 Li N, He Y, Chen Y, et al. Production of cyclic adenosine-3′,5′-monophosphate by whole cell catalysis using recombinant Escherichia coli, overexpressing adenylate cyclase[J]. Korean Journal of Chemical Engineering, 2013, 30(4):913-917. 3 Andexer J N, Richter M. Emerging Enzymes for ATP Regeneration in Biocatalytic Processes[J]. Chembiochem A European Journal of Chemical Biology, 2015, 16(3):380.

3. 毕业设计（论文）进程安排

2018.01-2018.01 阅读文献，完成开题报告 2018.02-2018.03 重组酶的表达与纯化 2018.04-2018.04 多酶催化体系的构建与优化 2018.05-2018.06 撰写毕业论文