1. 毕业设计（论文）的内容和要求

1．文献查阅 掌握文献查阅的一般方法，学会使用计算机在中国期刊网，维普数据库，超星数字图书馆，elsevier、ncbi电子期刊，springer link全文电子期刊，pubmed等检索资源上查阅关于工厂设计的相关文献。

2．文献阅读及综述 阅读与课题相关的外文及中文文献，了解国内外的研究动态，撰写文献综述。

3．明确实验任务，拟定实验方案 根据所查阅文献的内容，确定实验内容及方案，拟定科学可行的研究计划。

2. 参考文献

[1] Magenis RB, Prudencio ES, Molognoni L, Daguer H. A Control Method To Inspect the Compositional Authenticity of Minas Frescal Cheese by Gel Electrophoresis. J Agr Food Chem. 2014; 62: 8333-8339. [2]Wijayanti HB, Waanders J, Bansal N, Deeth HC. Identification of the binding of beta-lactoglobulin (beta-Lg) with sulfhydryl (-SH) blocking reagents by polyacrylamide gel electrophoresis (PAGE) and electrospray ionisation/time of flight-mass spectrometry (ESI/TOF-MS). Lwt-Food Sci Technol. 2015; 63: 934-938. [3] Wang Y, Wu LQ, Duan F, Yang B, Yang Y, Wang J. Development of an SI-Traceable HPLC-Isotope Dilution Mass Spectrometry Method To Quantify beta-Lactoglobulin in Milk Powders. J Agr Food Chem. 2014; 62: 3073-3080. [4] Costa FF, Brito MAVP, Furtado MAM, Martins MF, de Oliveira MAL, Barra PMD, Garrido LA, dos Santos ASD. Microfluidic chip electrophoresis investigation of major milk proteins: study of buffer effects and quantitative approaching. Anal Methods-Uk. 2014; 6: 1666-1673. [5] Crevillen AG, de Frutos M, Diez-Masa JC. On-chip single column transient isotachophoresis with free zone electrophoresis for preconcentration and separation of alpha-lactalbumin and beta-lactoglobulin. Microchem J. 2017; 133: 600-606. [6]He SF, Li X, Gao JY, Tong P, Chen HB. Development of sandwich ELISA for testing bovine beta-lactoglobulin allergenic residues by specific polyclonal antibody against human IgE binding epitopes. Food Chem. 2017; 227: 33-40. [7]Standardization IOf. Liquid milk -- Determination of acid-soluble beta-lactoglobulin content -- Reverse-phase HPLC method. ISO 13875. 2005; IDF 178:2005. [8]ADMINISTRATION USFD. Approaches to Establish Thresholds for Major Food Allergens and for Gluten in Food. Allergens. 2005. [9] Lepski S, Brockmeyer J. Impact of dietary factors and food processing on food allergy. Molecular nutrition food research, 2013, 57(1): 145-152. [10] Sponton O E, Perez A A, Carrara C, et al. Effect of limited enzymatic hydrolysis on linoleic acid binding properties of β-lactoglobulin. Food chemistry, 2014, 146: 577-582. [11] Vasilescu A, Nunes G, Hayat A, Latif U, Marty JL. Electrochemical Affinity Biosensors Based on Disposable Screen-Printed Electrodes for Detection of Food Allergens. Sensors-Basel. 2016; 16. [12] Buter L, Faber H, Wigger T, Vogel M, Karst U. Differential Protein Labeling Based on Electrochemically Generated Reactive Intermediates. Anal Chem. 2015; 87: 9931-9938. [13] Montiel VRV, Campuzano S, Conzuelo F, Torrente-Rodriguez RM, Gamella M, Reviejo AJ, Pingarron JM. Electrochemical magnetoimmunosensing platform for determination of the milk allergen beta-lactoglobulin. Talanta. 2015; 131: 156-162. [14] Boitz LI, Fiechter G, Seifried RK, Mayer HK. A novel ultra-high performance liquid chromatography method for the rapid determination of beta-lactoglobulin as heat load indicator in commercial milk samples. J Chromatogr A. 2015; 1386: 98-102. [15] Bu G, Luo Y, Zhang Y, et al. Effects of fermentation by lactic acid bacteria on the antigenicity of bovine whey proteins. Journal of the Science of Food and Agriculture, 2010, 90(12): 2015-2020. [16] Zhang Y, Tan C, Zhang X, et al. Effects of maltodextrin glycosylation following limited enzymatic hydrolysis on the functional and conformational properties of soybean protein isolate. European Food Research and Technology, 2014, 238(6): 957-968.

3. 毕业设计（论文）进程安排

2018.1.2-2018.1.13 文献查询和资料阅读 2018.1.13-2018.2.13 研究方案制定和开题报告写作 2018.2.14-2018.3.10 实践内容的准备 2018.3.11-2018.5.10 实践 2018.5.10-2018.5.15 实践内容处理与结果分析 2018.5.16-2018.6.1 毕业论文书写