论文总字数：16619字

摘 要

本实验通过制备了一种多孔温敏性葡聚糖基水凝胶，并且对该水凝胶的降解性、收缩性、回复性、释放性能、红外光谱及力学性能进行了研究。实验表明，葡聚糖基水凝胶为易降解的生物基材料；在35 ℃时发生剧烈收缩，在30 ℃下的回复率较高；葡聚糖基水凝胶与普通水凝胶相比，机械强度明显增强。通过活性污泥的混菌降解，第8天的降解率已超过30%，是一种易降解材料。红外光谱显示葡聚糖基水凝胶兼具葡聚糖和水凝胶的特征峰。

关键词：葡聚糖 水凝胶 降解材料 葡聚糖基水凝胶

Preparation of porous dextran-based hydrogels and its application in breathable dressings

ABSTRACT

In this experiment, a porous temperature-sensitive glucan-based hydrogel was prepared, and the degradability, shrinkage, recovery, release properties, infrared spectrum and mechanical properties of the hydrogel were studied. Experiments show that dextran-based hydrogels are easily biodegradable bio-based materials; they shrink dramatically at 35 °C and have a high recovery rate at 30 °C; dextran-based hydrogels are comparable to ordinary hydrogels. Mechanical strength was significantly enhanced. Through the mixed bacteria degradation of activated sludge, the degradation rate on the 8th day has exceeded 30%, which is an easily degradable material. Infrared spectra showed that the glucan-based hydrogel had both characteristic peaks of dextran and hydrogel.

Keywords: Dextran Hydrogels Degradable material Dextran-based hydrogel

目 录

摘要…………………………………………………………………………………I

ABSTRACT………………………………………………………………………II

第一章 绪论………………………………………………………………………1

1.1 前言………………………………………………………………………1

1.2 水凝胶的应用……………………………………………………………1

1.3 葡聚糖的生产及应用……………………………………………………2

1.4 研究思路和技术方法……………………………………………………3

第二章 葡聚糖的获得…………………………………………………………4

2.1 实验仪器与实验试剂……………………………………………………4

2.1.1 实验仪器……………………………………………………………4

2.1.2 实验试剂……………………………………………………………5

2.2 酶的获取…………………………………………………………………5

2.2.1 接种…………………………………………………………………5

2.2.2 培养…………………………………………………………………5

2.2.3 收集菌体……………………………………………………………5

2.2.4 破碎…………………………………………………………………6

2.2.5 收集酶液……………………………………………………………6

2.3 产葡聚糖…………………………………………………………………6

2.3.1 酶解…………………………………………………………………6

2.3.2 提取…………………………………………………………………6

2.3.3 纯化…………………………………………………………………6

2.3.4 真空冷冻干燥………………………………………………………6

第三章 葡聚糖基水凝胶………………………………………………………7

3.1 实验仪器与实验试剂……………………………………………………7

3.1.1 实验仪器……………………………………………………………7

3.1.2 实验试剂……………………………………………………………7

3.2 制备………………………………………………………………………7

3.3 特性………………………………………………………………………8

第四章 葡聚糖基水凝胶的性能测试…………………………………………9

4.1 降解………………………………………………………………………9

4.1.1 实验仪器与实验试剂……………………………………………9

4.1.2 实验方法…………………………………………………………9

4.1.3 实验结果…………………………………………………………10

4.2 收缩………………………………………………………………………11

4.2.1 实验仪器与实验试剂……………………………………………11

4.2.2 实验方法…………………………………………………………12

4.2.3 实验结果…………………………………………………………13

4.3 回复………………………………………………………………………14

4.3.1 实验仪器与实验试剂……………………………………………14

4.3.2 实验方法…………………………………………………………14

4.3.3 实验结果…………………………………………………………14

4.4 释放………………………………………………………………………15

4.4.1 实验仪器…………………………………………………………15

4.4.2 实验试剂…………………………………………………………16

4.4.3 BSA标准曲线的绘制……………………………………………16

4.4.4 实验方法…………………………………………………………17

4.4.5 实验结果…………………………………………………………18

4.5 红外光谱…………………………………………………………………19

4.6 力学性能测试分析………………………………………………………20

第五章 结语与展望……………………………………………………………21

5.1 结语………………………………………………………………………21

5.2 展望………………………………………………………………………21

参考文献…………………………………………………………………………22

致谢………………………………………………………………………………24

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