1. 毕业设计（论文）的内容和要求

论文内容本课题以实验室筛得的粪产杆菌来源的青霉素酰化酶g为对象，通过发酵破碎浓缩得到粗酶液。

以碳纤维支撑的聚乳酸（c-pla）为载体，通过食人鱼溶液处理，再使用硅烷偶联剂对其表面进行活性改性，向载体表面引入氨基基团，使得青霉素g酰化酶可以通过共价结合固定在载体上，最后利用固定化酶合成阿莫西林。

通过对本课题的研究可以为工业化酶法合成β-内酰胺类抗生素提供研究思路，获得形貌可控，机械结构稳定，担载量大的3d打印固定化载体，使得固定化酶在灵活适应各式反应器结构和工艺参数方面成为可能，大大降低后续分离的成本，也增加了效率。

2. 参考文献

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3. 毕业设计（论文）进程安排

起讫日期 设计（论文）各阶段工作内容 备 注 2018.12．28~2019.1.16 进行课题的调研和文献查阅、相关实验技能学习 2019.3.3~2019.3.30 修饰及制备3D打印载体，进行酶的固定化 2019.4.1~2019.4.30 酶学性质的测定 2019.5.1~2019.5.29 β-内酰胺类抗生素的合成 2019.5.31~2019.6.7 毕业论文撰写、答辩