1. 毕业设计（论文）的内容和要求

1．文献查阅 掌握文献查阅的一般方法，学会使用计算机在中国期刊网、维普数据库、超星数字图书馆等中文数据库、ca、sci等外文数据库检索资源上查阅有关文献。

2．文献阅读及综述 阅读与课题相关的外文及中文文献，了解解脂耶氏酵母胞质中乙酰-coa含量对菌体产油脂和3-羟基丙酸情况的影响，撰写文献综述。

3．明确实验任务，拟定实验方案 根据所查阅文献的内容，确定实验内容及方案，拟定科学可行的研究计划。

2. 参考文献

Bao Z., HamediRad M., Xue P., Xiao H., Tasan I., Chao R., et al. (2018). Genome-scale engineering of Saccharomyces cerevisiae with single nucleotide precision. Nat. Biotechnol. 36 505#8211;508. 10.1038/nbt.4132 [PubMed] [CrossRef] Borodina I., Kildegaard K. R., Jensen N. B., Blicher T. H., Maury J., Sherstyk S., et al. (2015). Establishing a synthetic pathway for high-level production of 3-hydroxypropionic acid in Saccharomyces cerevisiae via β-alanine. Metab. Eng. 27 57#8211;64. 10.1016/j.ymben.2014.10.003 [PubMed] [CrossRef] Bro C., Regenberg B., F#246;rster J., Nielsen J. (2006). In silico aided metabolic engineering of Saccharomyces cerevisiae for improved bioethanol production. Metab. Eng. 8 102#8211;111. 10.1016/j.ymben.2005.09.007 [PubMed] [CrossRef] Burk M. J., Osterhout R. E. (2010). Methods and organisms for production of 3-hydroxypropionic acid. U.S. Patent NO 20100021978. Chen X., Gao C., Guo L., Hu G., Luo Q., Liu J., et al. (2018a). DCEO biotechnology: tools to design, construct, evaluate, and optimize the metabolic pathway for biosynthesis of chemicals. Chem. Rev. 118 4#8211;72. 10.1021/acs.chemrev.6b00804 [PubMed] [CrossRef] Chen X., Yang X., Shen Y., Hou J., Bao X. (2018b). Screening phosphorylation site mutations in yeast acetyl-CoA carboxylase using malonyl-CoA sensor to improve malonyl-CoA-derived product. Front. Microbiol. 9:47. 10.3389/fmicb.2018.00047 [PMC free article] [PubMed] [CrossRef] Chen X., Yang X., Shen Y., Hou J., Bao X. (2017). Increasing malonyl-CoA derived product through controlling the transcription regulators of phospholipid synthesis in Saccharomyces cerevisiae. ACS Synth. Biol. 6 905#8211;912. 10.1021/acssynbio.6b00346 [PubMed] [CrossRef] Chen Y., Bao J., Kim I. K., Siewers V., Nielsen J. (2014). Coupled incremental precursor and co-factor supply improves 3-hydroxypropionic acid production in Saccharomyces cerevisiae. Metab. Eng. 22 104#8211;109. 10.1016/j.ymben.2014.01.005 [PubMed] [CrossRef] Chen Y., Daviet L., Schalk M., Siewers V., Nielsen J. (2013). Establishing a platform cell factory through engineering of yeast acetyl-CoA metabolism. Metab. Eng. 15 48#8211;54. 10.1016/j.ymben.2012.11.002 [PubMed] [CrossRef] Chen Y., Nielsen J. (2016). Biobased organic acids production by metabolically engineered microorganisms. Curr. Opin. Biotechnol. 37 165#8211;172. 10.1016/j.copbio.2015.11.004 [PubMed] [CrossRef] Chen Y., Siewers V., Nielsen J. (2012). Profiling of cytosolic and peroxisomal acetyl-CoAmetabolism in Saccharomyces cerevisiae. PLoS One 7:e42475. 10.1371/journal.pone.0042475 [PMC free article] [PubMed] [CrossRef] Daniel R., Bobik T. A., Gottschalk G. (1998). Biochemistry of coenzyme B12-dependent glycerol and diol dehydratases and organization of the encoding genes. FEMS Microbiol. Rev. 22 553#8211;566. 10.1111/j.1574-6976.1998.tb00387.x [PubMed] [CrossRef] David F., Nielsen J., Siewers V. (2016). Flux control at the malonyl-CoA node through hierarchical dynamic pathway regulation in Saccharomyces cerevisiae. ACS Synth. Biol. 5 224#8211;233. 10.1021/acssynbio.5b00161 [PubMed] [CrossRef] Gokarn R. R., Selifonova O. V., Jessen H. J., Gort S. J., Selmer T., Buckel W. (2011). 3-hydroxypropionic acid and other organic compounds. U.S. Patent No 8076120. Guo Z. P., Zhang L., Ding Z. Y., Shi G. Y. (2011). Minimization of glycerol synthesis in industrial ethanol yeast without influencing its fermentation performance. Metab. Eng. 13 49#8211;59. 10.1016/j.ymben.2010.11.003 [PubMed] [CrossRef]

3. 毕业设计（论文）进程安排

1.10-3.1 确定毕业设计的课题及相关资料的查找 3.2-3.10 实验方案的设计 3.10-3.20 实验材料的准备 3.21-5.15 准备分子实验 5.15-6.5 实验数据处理与结果分析 5.20-6.10 毕业论文书写