1. 毕业设计（论文）的内容和要求

一、论文内容： 线性α-烯烃（lao）：通常是指c4及c4以上直链端烯烃。

是一种重要的有机化工原料和中间体，被广泛用于高级润滑油基础油的合成、润滑油添加剂合成、烯烃共聚单体、以及清洁剂、乳化剂、浮选剂、粘合剂等，大于c12的线型α-烯烃是十分重要的合成精细有机化学品的原料，目前我国也完全依赖进口，具有很好的市场前景。

目前，生产线性-烯烃集中于化学法，但是化学法有着诸多的缺点目前，在催化脂肪酸脱羧生产脂肪烃的化学工业中，反应需高温、高压等极端条件，并且需使用贵金属催化剂，成本高昂且回收困难，其应用受到了极大的限制而生物法有着很大的优点：1.底物脂肪酸来源广泛，可以解决”地沟油问题”，2.反应条件温和，和化学法相比安全系数高。

2. 参考文献

[1] Yan J, Liu Y, Wang C, et al. Assembly of lipase and P450 fatty acid decarboxylase to constitute a novel biosynthetic pathway for production of 1-alkenes from renewable triacylglycerols and oils[J]. Biotechnology for biofuels, 2015, 8(1): 34.. [2] Grant J L, Mitchell M E, Makris T M. Catalytic strategy for carbon#8722; carbon bond scission by the cytochrome P450 OleT[J]. Proceedings of the National Academy of Sciences, 2016, 113(36): 10049-10054. [3] Grant J L, Hsieh C H, Makris T M. Decarboxylation of fatty acids to terminal alkenes by cytochrome P450 compound I[J]. Journal of the American Chemical Society, 2015, 137(15): 4940-4943.. [4] Bharadwaj V S, Kim S, Guarnieri M T, et al. Different Behaviors of a Substrate in P450 Decarboxylase and Hydroxylase Reveal Reactivity-Enabling Actors[J]. Scientific reports, 2018, 8(1): 12826. [5] Du J, Liu L, Guo L Z, et al. Molecular basis of P450 OleTJE: an investigation of substrate binding mechanism and major pathways[J]. Journal of computer-aided molecular design, 2017, 31(5): 483-495. [6] Faponle A S, Quesne M G, de Visser S P. Origin of the Regioselective Fatty‐Acid Hydroxylation versus Decarboxylation by a Cytochrome P450 Peroxygenase: What Drives the Reaction to Biofuel Production?[J]. Chemistry#8211;A European Journal, 2016, 22(16): 5478-5483. [7] Xu H, Ning L, Yang W, et al. In vitro oxidative decarboxylation of free fatty acids to terminal alkenes by two new P450 peroxygenases[J]. Biotechnology for biofuels, 2017, 10(1): 208. [8] Kourist R. A New Class of Enzymes Discovered: A Non‐Heme Oxidase Produces Medium‐Chain 1‐Alkenes[J]. Angewandte Chemie International Edition, 2015, 54(14): 4156-4158. [9] Lee J W, Niraula N P, Trinh C T. Harnessing a P450 fatty acid decarboxylase from Macrococcus caseolyticus for microbial biosynthesis of odd chain terminal alkenes[J]. Metabolic engineering communications, 2018, 7: e00076.

3. 毕业设计（论文）进程安排

起讫日期 设计（论文）各阶段工作内容 2018.12.12-12.18 选题，与导师讨论题目 2018.12.19 导师下达任务书 2018.12.20-2019.1.10 根据任务书要求，查找相关文献资料，完成任务书 2019.01.11-2019.01.23 提交开题报告 2019.01.23-05.23 完成试验并提交论文第一稿 2019.05.24-05.27 根据导师意见修改论文第一稿，完成并提交第二稿 2019.05.28-06.1 根据导师意见修改论文第二稿，完成并提交第三稿 2019.06.1-06.7 完成论文的审阅，准备答辩