1. 毕业设计（论文）的内容和要求

一、论文内容： 为了缓解环境能源危机，寻求可持续的替代能源刻不容缓[1]。

目前，一碳化合物的高效利用是当前生物制造领域的重要研究热点[2]。

其中以甲醇为原料受人关注，甲醇具有以下优势：含有更多电子，能够提供充足还原力；来源广泛，可从工业废气或煤炭等较多可再生资源中高效合成；成本较低，运输便捷，供应不因季节和气候而影响[3-5 ]。

2. 参考文献

[1] Liew F, Henstra A M, Kopke M, et al. Metabolic engineering of Clostridium autoethanogenum for selective alcohol production [J]. Metabolic Engineering, 2017, 40(104-14). [2] 王欣, 许宏贤, 周鹏,等. 不同原料生产赖氨酸的研究进展[J]. 食品与发酵工业, 2013, 39(10):174-180. [3] Olah G A. Beyond oil and gas: The methanol economy [J]. Angewandte Chemie-International Edition, 2005, 44(18): 2636-9. [4] Stacheter A, Noll M, Lee C K, et al. Methanol oxidation by temperate soils and environmental determinants of associated methylotrophs [J]. Isme Journal, 2013, 7(5): 1051. [5] Shen G J , Annous B A , Lovitt R W , et al. Biochemical route and control of butyrate synthesis in Butyribacterium methylotrophicum[J]. Applied Microbiology Biotechnology, 1996, 45(3):355-362. [6] Annous B A , Shieh J S , Shen G J , et al. Regulation of hydrogen metabolism in Butyribacterium methylotrophicum, by substrate and pH[J]. Applied Microbiology and Biotechnology, 1996, 45(6):804-810. [7] Lynd L H , Kerby R , Zeikus J G . Carbon monoxide metabolism of the methylotrophic acidogen Butyribacterium methylotrophicum[J]. Journal of Bacteriology, 1982, 149(1):255-263. [8] Dusch, N., A. Puhler and J. Kalinowski, Expression of the Corynebacterium glutamicum panD gene encoding L-aspartate-alpha-decarboxylase leads to pantothenate overproduction in Escherichia coli. APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1999. 65(4): p. 1530-1539. [9] Kerby R , Zeikus J G . Anaerobic catabolism of formate to acetate and CO2 by Butyribacterium methylotrophicum.[J]. Journal of Bacteriology, 1987, 169(5):2063. [10] O"Brien R W , Morris J G . Oxygen and the Growth and Metabolism of Clostridium acetobutylicum[J]. Journal of General Microbiology, 1971, 68(3):307-318.

3. 毕业设计（论文）进程安排

起讫日期 设计（论文）各阶段工作内容 2018.12.12-12.18 选题，与导师讨论题目 2018.12.19 导师下达任务书 2018.12.20-2019.1.10 根据任务书要求，查找相关文献资料 2019.01.11-2019.01.23 提交开题报告 2019.01.23-05.23 完成试验并提交论文第一稿 2019.05.24-05.27 根据导师意见修改论文第一稿，完成并提交第二稿 2019.05.28-06.1 根据导师意见修改论文第二稿，完成并提交第三稿 2019.06.1-06.7 完成论文的审阅，准备答辩