利用非氧化糖酵解途径提高碳利用效率的研究任务书
2020-05-22 21:10:48
1. 毕业设计(论文)的内容和要求
1.文献查阅
掌握文献查阅,使用网络资源如中国期刊网、维普数据库、超星数字图书馆、elsevier电子期刊、springer link全文电子期刊等检索资源,查阅关于非氧化糖酵解途径关键酶的表达相关文献。
2.文献阅读及综述
2. 参考文献
[1] marta papini, intawat nookaew, verena siewers, jens nielsen. physiological characterization of recombinant saccharomyces cerevisiae expressing the aspergillus nidulans phosphoketolase pathway: validation of activity through 13c-based metabolic flux analysis. applied microbiology and biotechnology,2012,4(95):1001-1010
[2] igor w. bogorad, tzu-shyang lin,james c. liao. synthetic non-oxidative glycolysis enables complete carbon conservation. nature,502,693#8211;697
[3] soo yun moon, soon ho hong, tae yong kima, sang yup lee. metabolic engineering of escherichia coli for the production of malic acid. biochemical engineering journal, 2008,2(40) :312#8211;320
3. 毕业设计(论文)进程安排
2015.12-2016.2 |
文献查阅,完成开题报告,熟悉实验仪器。 |
2016.3-2016.4 |
糖基转移酶产生菌株鉴定 |
2016.4-2016.5 |
糖基转移酶基因克隆 |
2016.5-2016.6 |
糖基转移酶表达载体构建 |
2016.6-2016.6 |
糖基转移酶表达条件优化 |