细菌胞外还原四价硒的研究毕业论文
2021-06-24 22:05:28
摘 要
Abstract...........................................................................................................................................ii
第一章 绪论...................................................................................................................................1
1.1硒污染的来源.........................................................................................................................1
1.2硒污染的危害.........................................................................................................................1
1.3微生物还原修复.....................................................................................................................2
第二章 材料与方法.......................................................................................................................4
2.1仪器与试剂.............................................................................................................................4
2.1.1仪器....................................................................................................................................4
2.1.2其他实验用具....................................................................................................................4
2.1.3试剂....................................................................................................................................5
2.1.4培养基与试剂制备............................................................................................................5
2.2实验方法.................................................................................................................................6
2.2.1四价硒的测定方法............................................................................................................6
2.2.2粗提物提取及总蛋白含量测定........................................................................................7
2.2.3四价硒还原酶活性定位....................................................................................................9
2.2.4起始浓度对无细胞提取物还原四价硒的影响........................... ...................................10
2.2.5电子供体对无细胞提取物还原四价硒的影响...............................................................10
2.2.6金属离子对无细胞提取物还原四价硒的影响...............................................................11
2.2.7亚硒酸盐还原酶的动力学常数.......................................................................................11
- 结果与分析......................................................................................................................11
3.1硒的标准曲线........................................................................................................................11
3.2蛋白的标准曲线和含量测定................................................................................................12
3.3确定四价硒还原酶的细胞定位............................................................................................13
3.4起始浓度对无细胞提取物还原四价硒的影响....................................................................14
3.5电子供体对无细胞提取物还原四价硒的影响....................................................................15
3.6金属离子对无细胞提取物还原四价硒的影响....................................................................17
3.7亚硒酸盐还原酶的动力学常数............................................................................................18
- 讨论..................................................................................................................................19
- 结论..................................................................................................................................22
参考文献........................................................................................................................................23
致谢................................................................................................................................................25
