全文总字数：2241字

1. 1. 毕业设计（论文）的内容、要求、设计方案、规划等

一、前言

通过查阅文献了解ice基因的发现、功能、分子作用机理与研究概况，熟练掌握分子实验各种仪器操作，以及rna提取、pcr、凝胶电泳与race等分子克隆相关技术方法。

二、方案拟定

2. 参考文献（不低于12篇）

[1]michael f thomashow, sarah j gilmour, eric j stockinger, et al. role of the arabidopsis cbf transcriptional activators in cold acclimation [j]. physiol plant. 2001,112:171~175. 767~781

[2]gilmour s j, zarka d g, stockinger e j, et al. low temperature regulation of the arabidopsis cbf family of ap2 transcriptional activators as an early step in cold-induced cor gene expression[j].the plant journal, 1998, 16(4): 433-442.

[3]chinnusamy v, ohta m, kanrar s, etal. ice1: a regulator of cold-induced transcription and freezing tolerance in arabidopsis[j].genes development, 2003, 17: 1043-1054.