1. 毕业设计（论文）的内容和要求

1、采用计算机检索20篇中文文献和5篇英文文献； 2、阅读20篇文献，翻译一篇英文文献，提交一篇开题报告。

格式要求为：参考文献标注符合规范，计算机打印； 3、实验工作主要内容 （1）以白色链霉菌s.albulus pd-1为研究对象，探究添加前提赖氨酸对其聚赖氨酸产量的影响； （2）过表达天冬氨酸激酶基因，解除赖氨酸对天冬氨酸激酶的反馈抑制，从而进一步提高 ε-pl 的产量； （3）进行发酵实验，检测对赖氨酸的利用率，以及优化赖氨酸添加的方式。

4、中期报告 以powerpoint制作中期报告，必须对课题的研究背景、文献分析的现及初 步实验结果进行汇报。

2. 参考文献

[1]. Shima S, Sakai H. 1977. Polylysine produced by Streptomyces. Agric. Biol. Chem. 41: 1807-1809. [2]. Yoshida, T., Nagasawa, T., 2003. ε-Poly-L-lysine: microbial production, biodegradation and application potential. Appl. Microbiol. Biotechnol. 62, 21-26. [3]. Hiraki J, Hatakeyama M, Morita H, et al. Improved curly epsilon-Poly-L-Lysine Production of an S-(2-Aminoethyl)-L-Cysteine Resistant Mutant of Streptomyces albulus[J]. Seibutsu Kogaku Kaishi, 1998, 76: 487-493. [4]. Kahar P, Iwata T, Hiraki J, et al. Enhancement of ε-polylysine production by Streptomyces albulus strain 410 using pH control. Journal of bioscience and bioengineering, 2001, 91(2): 190-194. [5]. Guoliang Wang，Shiru Jia，Tian Wang，Liye Chen， Qingchao Song， Weina Li. Effect of Ferrous Ion on e-Poly-L-Lysine Biosynthesis by Streptomyces diastatochromogenes CGMCC3145. Curr Microbiol (2011) 62:1062#8211;1067 [6]. Zhang Y, Feng X, Xu H, et al. ε-Poly-L-lysine production by immobilized cells of Kitasatospora sp. MY 5-36 in repeated fed-batch cultures. Bioresource technology, 2010, 101(14): 5523-5527. [7]. Jun Xia ，Hong Xu ， Xiaohai Feng， Zhaoxian Xu Poly(L-diaminopropionic acid), a novel non-proteinic amino acid oligomer co-produced with poly(ε-L-lysine)by Streptomyces albulus PD-1. Appl Microbiol Biotechnol, 2013 97:7597#8211;7605 [8] Yamanaka K, Maruyama C, Takagi H, et al. ε-poly-L-lysine dispesity is controlled by a highly unusual non-ribosomal peptide synthetase. Natural Chemical Biology, 2008, 4(12): 766-772. [9]. Jan C M van Hest. Flexible peptide assembly[J]. Nature, 2008, 456(13): 186-187. [10]. Fortin P D, Walsh C T, Magarvey N A. A transglutaminase homologue as a condensation catalyst in antibiotic assembly lines [J].Nature, 2007, 448: 824-828. [11]. Sieber S A, Marahiel M A. Molecular mechanisms underlying nonribosomal peptide synthesis: approaches to new antibiotics [J]. Chemical Reviews, 2005,105: 715-738. [12]. Shima S, Sakai H. 1977. Polylysine produced by Streptomyces. Agric. Biol. Chem. 41: 1807-1809. [13]Yoshida, T., Nagasawa, T., 2003. ε-Poly-L-lysine: microbial production, biodegradation and application potential. Appl. Microbiol. Biotechnol. 62, 21#8211;26. [14]. Hiraki J, Hatakeyama M, Morita H, et al. Improved curly epsilon-Poly-L-Lysine Production of an S-(2-Aminoethyl)-L-Cysteine Resistant Mutant of Streptomyces albulus[J]. Seibutsu Kogaku Kaishi, 1998, 76: 487-493. [15]. Kahar P, Iwata T, Hiraki J, et al. Enhancement of ε-polylysine production by Streptomyces albulus strain 410 using pH control. Journal of bioscience and bioengineering, 2001, 91(2): 190-194. [16]. Guoliang Wang，Shiru Jia ，Tian Wang，Liye Chen， Qingchao Song， Weina Li. Effect of Ferrous Ion on e-Poly-L-Lysine Biosynthesis by Streptomyces diastatochromogenes CGMCC3145. Curr Microbiol (2011) 62:1062#8211;1067 [17].Zhang Y, Feng X, Xu H, et al. ε-Poly-L-lysine production by immobilized cells of Kitasatospora sp. MY 5-36 in repeated fed-batch cultures. Bioresource technology, 2010, 101(14): 5523-5527. [18].Jun Xia ，Hong Xu ， Xiaohai Feng， Zhaoxian Xu Poly(L-diaminopropionic acid), a novel non-proteinic amino acid oligomer co-produced with poly(ε-L-lysine)by Streptomyces albulus PD-1. Appl Microbiol Biotechnol (2013) 97:7597#8211;7605 [19]. 陈旭升 .Streptomyces sp.M-Z18发酵生产ε-聚赖氨酸 [20].Chen X, Tang L, Li S, et al. Optimization of medium for enhancement of ε-Poly-L-Lysine production by Streptomyces sp. M-Z18 with glycerol as carbon source. Bioresource technology, 2011, 102(2): 1727-1732. [21]. Shima, S., Sakai, H., 1981. Poly-L-lysine produced by Streptomyces. Part II. Taxonomy and fermentation studies. Agric. Biol. Chem. 45, 2497#8211;2502.

3. 毕业设计（论文）进程安排

起讫日期 设计（论文）各阶段工作内容 备 注 2016.10.10#8212;2016.10.29 确定论文题目、填写任务书 2016．10.30--2016.11.6 毕业设计（论文）任务书及进度表 2016.11.7--2017.1.15 查找资料、写开题报告 2017.1.16--2017.2.15 初步实验及验证 2017.2.16--2017.3.31 完善实验、数据处理 2017.4.1--2017.4.15 论文写作 2017.4.16--2017.4.25 完成论文初稿交老师批阅 2017.4.26--2017.5.15 论文修改