1. 毕业设计（论文）的内容和要求

1.文献查阅 学会利用计算机在中国期刊网、维普数据网、springerlink、ca、sci、elsevier等等网络检索资源上检索、查阅与紫外诱变有关的文献，掌握文献查阅的方法。

2.文献阅读及综述 仔细阅读与所做课题相关的国内外文献，了解相关的背景知识以及目前国内外的研究动态，会系统分析，撰写文献综述。

3.明确实验任务，拟定实验方案 根据所查文献的内容，结合大学所学课程相关知识，确定实验内容及方案，拟定科学可行的研究计划。

2. 参考文献

[1] Ashiuchi M. Microbial production and chemical transformation of poly-γ-glutamate. Microbial Biotechnology. 2013, 6: 664-674. [2] Li X, Gou X, Long D, Ji Z, Hu L, Xu D, Liu J and Chen S. Physiological and metabolic analysis of nitrate reduction on poly-γ-glutamic acid synthesis in Bacillus licheniformis WX-02. Archives of Microbiology. 2014, 196: 791-799. [3] Ashiuchi M, Soda K and Misono H. A poly-γ-glutamate synthetic system of Bacillus subtilis IFO 3336: gene cloning and biochemical analysis of poly-γ-glutamate produced by Escherichia coli clone cells. Biochemical and Biophysical Research Communications. 1999,263: 6-12 [4] Xu H, Jiang M, Li H, Lu D and Ouyang P. Efficient production of poly(γ-glutamic acid) by newly isolated Bacillus subtilis NX-2. Process Biochemistry. 2005, 40: 519-523 [5] Cao M, Geng W, Liu L, Song C, Xie H, Guo W, Jin Y and Wang S. Glutamic acid independent production of poly-γ-glutamic acid by Bacillus amyloliquefaciens LL3 and cloning of pgsBCA genes. Bioresource Technology. 2011, 102: 4251-4257. [6] Munoz-Dorado J, Inouye S and Inouye M. A gene encoding a protein serine/threonine kinase is required for normal development of M. xanthus, a gram-negative bacterium. Cell. 1991,67: 995-1006.

3. 毕业设计（论文）进程安排

起讫日期 设计（论文）各阶段工作内容 备 注 2016.10.10#8212;2016.10.29 确定论文题目、填写任务书 2016．10.30--2016.11.6 毕业设计（论文）任务书及进度表 2016.11.7--2016.12.6 查找资料、写开题报告 2016.12.7--2017.1.12 初步实验及验证 2017.3.1--2017.4.30 完善实验、数据处理 2017.4.1--2017.4.15 论文写作 2017.4.16--2017.4.25 完成论文初稿交老师批阅 2017.4.26--2017.5.15 论文修改 2017.5.16#8212;2017.6.1 论文答辩准备