1. 毕业设计（论文）的内容和要求

有研究表明，在发酵培养基中添加一定浓度的乙醇，可以显著提高BC的产量。

在醋酸菌发酵的过程中添加一定浓度的乙醇溶液，以细菌纤维膜的干湿重作为评价指标，考察乙醇浓度及添加量对细菌纤维素产量的影响，并寻找最佳的添加量；并通过检测发酵过程中菌体数量、细胞壁通透性、纤维素合成过程中关键酶的酶活等指标的变化，以期阐明乙醇促进醋酸菌产细菌纤维素的机理。

2. 参考文献

[1]BROWN A J. On an acetic ferment which forms cellulose [J]. J Chem Soc,1886, 49: 432439. [2]SHAN W, LIU H, LIU X, et al. Development of silver sulfadiazine loaded bacterial cellulose/sodium alginate composite films with enhanced antibacterial property[J]. Carbohydrate Polymers, 2015, 132:351-8. [3]KIRDPONPATTARA S, KHAMKEAW A, SANHAVANAKIT N, et al. Structural modification and characterization of bacterial cellulose#8211;alginate composite scaffolds for tissue engineering[J]. Carbohydrate Polymers, 2015, 132:146-155. [4]QIAO K, ZHENG Y, GUO S, et al. Hydrophilic nanofiber of bacterial cellulose guided the changes in the micro-structure and mechanical properties of nf-BC/PVA composites hydrogels[J]. Composites Science amp; Technology, 2015, 118:47-54. [5]HUANG C, YANG X Y, XIONG L, et al. Using wastewater after lipid fermentation as substrate for bacterial cellulose production by Gluconacetobacter xylinus[J]. Letters in Applied Microbiology,2015,136(5):198-202. [6]朱清梅, 冯玉红, 林强,等. 细菌纤维素改性研究进展[J]. 现代化工, 2009, 29(8):34-37. [7]MARTINEZ H, BRACKMANN C, ENEJDER A, et al. Mechanical stimulation of fibroblasts in micro-channeled bacterial cellulose scaffolds enhances production of oriented collagen fibers.[J]. Journal of Biomedical Materials Research Part A, 2012, 100(4):948#8211;957. [8]Sakairi N, Asano H, Ogawa M, et al. A method for direct harvest of bacterial cellulose filaments during continuous cultivation of Acetobacter xylinum[J]. Carbohydrate Polymers, 1998, 35(3):233-237. [9]Kouda T, Yano H, Yoshinaga F. Effect of agitator configuration on bacterial cellulose productivity in aerated and agitated culture[J]. Journal of Fermentation amp; Bioengineering, 1997, 83(4):371-376. [10]Jonas R, Farah L F. Production and application of microbial cellulose[J]. Polymer Degradation amp; Stability, 1998, 59(s 1#8211;3):101-106. [11]Borzani W, Souza S J D. Mechanism of the film thickness increasing during the bacterial production of cellulose on non-agitaded liquid media[J]. Biotechnology Letters, 1995, 17(11):1271-1272. [12]Jung J Y, Park J K, Chang H N. Bacterial cellulose production by Gluconacetobacter hansenii in an agitated culture without living non-cellulose producing cells[J]. Enzyme amp; Microbial Technology, 2005, 37(3):347-354. [13]Czaja W, Krystynowicz A, Bielecki S, et al. Microbial cellulose#8212;the natural power to heal wounds [J]. Biomaterials, 2006, 27(2):145-151. [14]Kouda T, Yano H, Yoshinaga F. Characterization of non-newtonian behavior during mixing of bacterial cellulose in a bioreactor[J]. Journal of Fermentation amp; Bioengineering, 1996, 82(4):382-386. [15]谭丽丽. 响应面法优化红茶菌细菌纤维素的合成[D]. 安徽农业大学, 2012. [16]周伶俐. 细菌纤维素生产菌的筛选、发酵及应用的研究[D]. 南京理工大学, 2008.

3. 毕业设计（论文）进程安排