1. 毕业设计（论文）的内容和要求

1、采用计算机检索20篇中文文献和5篇英文文献； 2、阅读20篇文献，翻译一篇英文文献，提交一篇开题报告。

格式要求为：参考文献标注符合规范，计算机打印； 3、实验工作主要内容 （1）查阅酶催化法生产α-熊果苷相关文献及掌握基因工程菌株构建的方法； （2）构建蔗糖磷酸化酶基因的表达质粒，并在大肠杆菌中验证其功能； （3）全细胞催化法生产α-熊果苷及转化条件的优化。

4、中期报告 以powerpoint制作中期报告，必须对课题的研究背景、文献分析的现及初 步实验结果进行汇报。

2. 参考文献

[1]王慧琛,王丽琴. 熊果苷的应用及检测研究进展[J].天津药学,2012,(04):71-74. [2]张凤兰,黄湘鹭,曹进,徐潇,王钢力,崔生辉. 熊果苷的遗传毒性及人体表皮细菌对熊果苷代谢转化作用研究[J].中国药事,2014,(04):375-380. [3]秦良,刘有停,陈毅明,张鹏. 基础环境因素对α-熊果苷和β -熊果苷稳定性的影响[J]. 日用化学工业,2014,(10):580-583. [4]彭颖. 全细胞反应器生产α-熊果苷[D].山东大学,2015. [5]郭起,陈朗秋,蔡进,史慎德,刘永青. 熊果苷的合成新方法[J]. 化学试剂,2010,(01):17-20. [6]滕希峰,李钟,刘龙元,何洋,王亚青,陈星宇,梁颖欣,何琳. α-熊果苷平衡溶解度、表观油水分配系数和解离常数的测定[J]. 中成药,2016,(11):2366-2370. [7]赵如奎,吴剑荣,詹晓北,朱莉. α-环糊精葡萄糖基转移酶催化合成α-熊果苷[J]. 生物加工过程,2015,(04):36-41. [8]Synthesis of acyl derivatives of salicin, salirepin, and arbutin[J].Elena V. Stepanova,Maxim L. Belyanin,Victor D. Filimonov. Carbohydrate Research. 2014 [9]Toward a cost-effective method for α-arbutin production by using immobilized hydroquinone as a glucosyl acceptor[J].Chunqiao Liu,Li Deng,Peng Zhang,Shurong Zhang,Tao Xu,Fang Wang,Tianwei Tan.Process Biochemistry . 2013 (10) [10]Efficient production of α-arbutin by whole-cell biocatalysis using immobilized hydroquinone as a glucosyl acceptor[J].Chun-Qiao Liu,Li Deng,Peng Zhang,Shu-Rong Zhang,Tao Xu,Fang Wang,Tian-Wei Tan. Journal of Molecular Catalysis. B, Enzymatic.2013 [11]Isolation of α-arbutin from Xanthomonas CGMCC 1243 fermentation broth by macroporous resin adsorption chromatography[J].Chunqiao Liu,Li Deng,Peng Zhang,Luo Liu,Tao Xu,Tianwei Tan,Fang Wang. Journal of Chromatography B. 2013 [12]Rapid quantification of reducing sugars in biomass hydrolysates:Improving the speed and precision of the dinitrosalicylic acid assay[J].Ian P. Wood,Adam Elliston,Peter Ryden,Ian Bancroft,Ian N. Roberts,Keith W. Waldron. Biomass and Bioenergy. 2012 [13]Purification, characterization, and gene identification of an α-glucosyl transfer enzyme, a novel type α-glucosidase from Xanthomonas campestris WU-9701[J]. Toshiyuki Sato,Nobukazu Hasegawa,Jun Saito,Satoru Umezawa,Yuki Honda,Kuniki Kino,Kohtaro Kirimura. Journal of Molecular Catalysis. B, Enzymatic.2012 [14]Biotechnological production of arbutins (α-andβ-arbutins),skin-lightening agents, and their derivatives[J].Dong-Ho Seo,Jong-Hyun Jung,Jae-Eun Lee,Eun-Jung Jeon,Wooki Kim,Cheon-Seok Park.Applied Microbiology and Biotechnology.2012 (6) [15]High-yield enzymatic bioconversion of hydroquinone to α-arbutin, a powerful skin lightening agent, by amylosucrase[J].Dong-Ho Seo,Jong-Hyun Jung,Suk-Jin Ha,Hyun-Kug Cho,Dong-Hyun Jung,Tae-Jip Kim,Nam-In Baek,Sang-Ho Yoo,Cheon-Seok Park. Applied Microbiology and Biotechnology. 2012 (5) [16]Biosynthesis of ( )-catechin glycosides using recombinant amylosucrase from Deinococcus geothermalis DSM 11300[J] . Hyun-Kug Cho,Hee-Hang Kim,Dong-Ho Seo,Jong-Hyun Jung,Ji-Hae Park,Nam-In Baek,Myo-Jeong Kim,Sang-Ho Yoo,Jaeho Cha,Young-Rok Kim,Cheon-Seok Park. Enzyme and Microbial Technology . 2011 (2) [17]Arbutin:Isolation,X-ray structure and computional studies[J].Jacek E. Nycz,Grzegorz Malecki,Monika Morag,Gerard Nowak,Lukasz Ponikiewski,Joachim Kusz,Anna Switlicka. Journal of Molecular Structure.2010 (1)

3. 毕业设计（论文）进程安排

2016.10.10#8212;2016.10.29 确定论文题目、填写任务书 2016．10.30--2016.11.6 毕业设计（论文）任务书及进度表 2016.11.7--2017.1.15 查找资料、写开题报告 2017.1.16--2017.2.15 初步实验及验证 2017.2.16--2017.3.31 完善实验、数据处理 2017.4.1--2017.4.15 论文写作 2017.4.16--2017.4.25 完成论文初稿交老师批阅 2017.4.26--2017.5.15 论文修改 2017.5.16#8212;2017.6.1 论文答辩准备