论文总字数：19545字

摘 要

角鲨烯、AA及EPA是具有多种生理功能的多不饱和脂肪酸，其分离技术成为目前产品开发及利用的关键。本文基于亲和膜分离原理，在前期研究的陶瓷膜材料Ag -SACM（负载Ag 氧化铝陶瓷膜）基础上，采用角鲨烯、AA和EPA模拟体系，利用自行搭建的小型动态吸附实验装置，考察Ag -SACM对上述3种化合物的动态吸附性能。结果表明，当各组分体积流量为2.5 mL/s，初始浓度均为20 μg/mL时，Ag -SACM对角鲨烯、AA和EPA的吸附量分别为140 μg/m²、105 μg/m²和498 μg/m²。以EPA为目标物质，研究其洗脱条件，结果表明：乙酸丁酯对EPA的洗脱效果最好，在洗脱溶剂体积30 mL，温度70℃，洗脱时间75 min条件下，EPA的洗脱率可达90.1 %。以乙酸丁酯作为洗脱溶剂，在优化的实验条件下反复进行吸附-洗脱，考察Ag -SACM的重复使用性能。结果表明当重复使用5次时，EPA的洗脱率仍可达80 %以上。

关键词：氧化铝陶瓷膜 硝酸银 EPA 吸附性能 洗脱

Dynamic adsorption and elution of Ag -SACM for squalene, arachidonic acid and EPA

Abstract

Squalene, AA and EPA are a variety of physiological functions of polyunsaturated fatty acids, and separation technology has become the key to the current product development and utilization. Based on affinity membrane separation principle and the preliminary studies of ceramic membrane material Ag -SACM (loading Ag to alumina ceramic membrane).To further investigate potential Ag -SACM,we use squalene, AA and EPA model system to study Ag -SACM dynamic adsorption properties of the above three compounds by building a small set of dynamic adsorption experiment device and testing the dynamic adsorption performance of Ag -SACM to squalene,The results showed that when the initial concentration of components was 20 μg/mL, the volume flow was 2.5 mL/s, the adsorption quantity of the Ag -SACM to squalene, AA and EPA were 140 μg/m²、105 μg/m² and 498 μg/m².Choosing Ag -SACM as the target substance to study the elution conditions.Accoding to the results, the eluting effect of butyl acetate on EPA was the best. Under conditions that elution liquid volume of 30 mL, elution time of 75 min, elution temperature of 70 ℃, the EPA elution rate was 90.1%.Choosing butyl acetate as the solvent elution, operating the process of adsorption – elution repeatedly under the optimizing experimental conditions, finally investigating the reusability of Ag -SACM.The results showed,when repeatedly using the materials for 5 times, he EPA elution rate was more than 80%.

Keywords:Alumina ceramic membrane；Silver nitrate；EPA；Adsorption performance；Elution

目录

摘 要 I

Abstract II

第一章 文献综述 1

1.1 概述 1

1.1.1角鲨烯、AA及EPA的结构、性质及功能..............................................1

1.1.2 角鲨烯、AA及EPA的应用..................................................................2

1.2 角鲨烯、AA及EPA提取分离技术的研究进................................................ 3

1.2.1 分子蒸馏法 3

1.2.2 超临界流体萃取法 4

1.2.3 色谱分离法 .4

1.2.4低温结晶法...........................................................................................5

1.2.5银离子络合法.......................................................................................5

1.3陶瓷膜的性质及应用 6

1.4本文的研究目的和内容 .6

1.4.1 研究目的与意义....................................................................................6 1.4.2 主要研究内容.......................................................................................7

第二章 实验材料与方法 8

2.1 实验材料 8

2.1.1 实验试剂与仪器设备........................................................................8

2.2 实验方法 8

2.2.1角鲨烯和AA的分析检测方法............................................................8

2.2.2角鲨烯和AA标准曲线的绘制.............................................................9

2.2.3 EPA的分析检测方法...........................................................................9

2.2.4 EPA标准曲线的绘制...........................................................................9

2.2.5角鲨烯、AA及EPA在Ag -SACM上的动态吸附..............................9

2.2.6不同溶剂对EPA的洗脱效果..............................................................10

2.2.7 Ag -SACM的重复使用性能...............................................................11

第三章 实验结果与讨论 13

3.1 实验结果 13

3.1.1绘制角鲨烯、AA和EPA的标准曲线................................................13

3.1.2角鲨烯、AA及EPA在Ag -SACM上的动态吸附..............................14

3.1.3 EPA的洗脱.........................................................................................16

3.1.4Ag -SACM的重复使用性能................................................................18

3.2 讨论...............................................................................................................19

第四章 结论与展望 20

4.1 结论 20

4.2 展望 20

参考文献 22

致 谢 25

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