16S rRNA基因条形码在市售烤鱼片鱼类品种鉴定中的应用任务书
2020-07-02 23:00:13
1. 毕业设计(论文)的内容和要求
烤鱼片是采用鲜活的海水鱼类为原料,经过预处理、清洗、切片、漂洗、调制、水分沥干、干燥、焙烤轧松、整型、产品包装等流程制作而成的即食类预包装产品,含有较丰富的叶酸、泛酸、烟酸、多种维生素及磷、钙、铁等矿物质,是高营养、低胆固醇、易被机体吸收的营养食品。
由于其本身所具有的口感鲜美、肉质松软等优点,已然作为人们休闲、外出、野营、佐酒等时常食用的即食类零食而受到众多消费人群的青睐。
目前,市售烤鱼片的种类繁多,包括烤马面鲀片、鱿鱼片、烤黄花鱼片等。
2. 参考文献
[1] Wen, J., et al., An insight into the Chinese traditional seafood market: Species characterization of cephalopod products by DNA barcoding and phylogenetic analysis using COI and 16SrRNA genes. Food Control, 2017. 82: p. 333-342. [2] Xiong, X., et al., Multiple fish species identified from China's roasted Xue Yu fillet products using DNA and mini-DNA barcoding: Implications on human health and marine sustainability. Food Control, 2018. 88: p. 123-130. [3]. Chin, T.C., et al., Detection of mislabelled seafood products in Malaysia by DNA barcoding: Improving transparency in food market. Food Control, 2016. [4]. Hebert, P.D.N., S. Ratnasingham and J.R. de Waard, Barcoding animal life: cytochrome c oxidase subunit 1 divergences among closely related species. Proceedings of the Royal Society B: Biological Sciences, 2003. 270(Suppl_1): p. S96-S99. [5]. G#252;nther, B., M.J. Raupach and T. Knebelsberger, Full-length and mini-length DNA barcoding for the identification of seafood commercially traded in Germany. Food Control, 2017. 73: p. 922-929. [6]. Armani, A., et al., Development of a Simple and Cost-Effective Bead-Milling Method for DNA Extraction from Fish Muscles. Food Analytical Methods, 2014. 7(4): p. 946-955.
3. 毕业设计(论文)进程安排
1)2017年09月-2016年11月 查阅文献,完成外文翻译、文献综述和开题报告等工作; 2)2017年12月-2018年03月 完成课题的实验部分; 3)2018年04月-2018年05月 完成毕业论文写作,进行毕业设计的审阅和修改完善; 4)2018年06月 完成毕业论文的答辩资格审查、答辩;