1. 毕业设计（论文）的内容和要求

d-塔格糖是一种自然界罕见的己酮糖，它在结构上是d-半乳糖的异构体。

d-塔格糖具有多种独特的生理特性与功能，如低热量、降血糖、不致龋齿、改善肠道菌群等，近年来已被发达国家开发作为具有高经济附加值的功能性甜味剂进行销售。

d-塔格糖的商业化生产长期以来依赖化学催化法，随着上世纪九十年代利用l-阿拉伯糖异构酶（简称l-ai酶）催化d-半乳糖制备d-塔格糖技术的兴起，生物法生产d-塔格糖成为了新的发展趋势。

2. 参考文献

[1] Oh DK. Tagatose: properties, applications, and biotechnological processes [J]. Appl Microbiol Biotechnol, 2007, 76:1-8. [2] Beadle JR, Saunder JP, Wajada TJ. Process for manufacturing tagatose [P]. US Patent 500261, 1991. [3] Cheetham PSJ, Wootton AN. Bioconversion of D-galactose into D-tagatose [J]. Enzyme Microb Technol, 1993, 15:105-108. [4] Boudebbouze S, Maguin E, Rhimi M. Bacterial L-arabinose isomerases: industrial application for D-tagatose production [J]. Recent Pat DNA Gene Seq, 2011, 5(3):194-201. [5] Lu Y, Levin GV, Donner TW. Tagatose, a new antidiabetic and obesity control drug [J]. Diabetes Obes Metab, 2008, 10(2):109-134. [6] Kim JH, Prabhu P, Jeya M, et al. Characterization of an L-arabinose isomerase from Bacillus subtilis [J]. Appl Microbiol Biotechnol, 2009, 85(6):1839-1847. [7] Chouayekh H, Bejar W, Rhimi M, et al. Characterization of an l-arabinose isomerase from the Lactobacillus plantarum NC8 strain showing pronounced stability at acidic pH [J]. FEMS Microbiol Lett, 2007, 277:260-267. [8] Lee SJ, Lee DW, Choe EA, et al. Characterization of a thermoacidophilic l-arabinose isomerase from Alicyclobacillus acidocaldarius: role of Lys-269 in pH optimum [J]. Appl Environ Microbiol, 2005, 71:7888-7896.

3. 毕业设计（论文）进程安排

2018.10.10-2018.10.29 确定论文题目、填写任务书 2018.10.30-2018.11.6 毕业设计（论文）任务书及进度表 2018.11.7-2018.12.6 查找资料、写开题报告 2018.12.7-2019.2.12 初步实验及验证 2019.3.1-2019.3.30 完善实验、数据处理 2019.4.1-2019.4.15 论文写作 2019.4.16-2019.4.25 完成论文初稿交老师批阅 2019.4.26-2019.5.15 论文修改 2019.5.16-2019.6.1 论文答辩准备