1. 毕业设计（论文）的内容和要求

1．文献查阅 掌握文献查阅的一般方法，学会使用计算机在中国期刊网，维普数据库，超星数字图书馆，elsevier、ncbi电子期刊，springer link全文电子期刊，pubmed等检索资源上查阅关于脂肪酸和甾醇类成分衍生化及分析方法的相关文献。

2．文献阅读及综述 阅读与课题相关的外文及中文文献，了解国内外的研究动态，撰写文献综述。

3．明确实验任务，拟定实验方案 根据所查阅文献的内容，确定实验内容及方案，拟定科学可行的研究计划。

2. 参考文献

[1] F.Q. Yang, K. Feng, J. Zhao, S.P. Li, Analysis of sterols and fatty acids in natural and cultured Cordyceps by one-step derivatization followed with gas chromatography-mass spectrometry, J Pharm Biomed Anal 49 (2009) 1172-1178. [2] M.J. Lagarda, G. Garc#237;a-Llatas, R. Farr#233;, Analysis of phytosterols in foods, Journal of Pharmaceutical and Biomedical Analysis 41 (2006) 1486-1496. [3] C.W. Roberts, R. McLeod, D.W. Rice, M. Ginger, M.L. Chance, L.J. Goad, Fatty acid and sterol metabolism: potential antimicrobial targets in apicomplexan and trypanosomatid parasitic protozoa, Mol Biochem Parasitol 126 (2003) 129-142. [4] P.J. Wan, M.K. Dowd, A.E. Thomas, B.H. Butler, Trimethylsilyl derivatization/gas chromatography as a method to determine the free fatty acid content of vegetable oils, JAOCS, Journal of the American Oil Chemists' Society 84 (2007) 701-708. [5] Z.L. Wang Y. J., Ding A.W., Quantification of five kinds of organic acids in Ganoderma Lucidum Spores Oil by GC-MS, Journal of Nanjing University of Traditional Chinese Medicine 25 (2009) 136-139. [6] T.Q. Chen, X.Y. Zhao, J.Z. Wu, D.Y. Yu, Y.B. Wu, Supercritical fluid CO2 extraction, simultaneous determination of components in ultra-fine powder of Ganoderma sinense by HPLC-ESI-MS method, Journal of the Taiwan Institute of Chemical Engineers 42 (2011) 428-434. [7] J.Z. Dang, C.M. Ning, H.Q. Wu, L.X. Feng, Y.Y. Ye, Study on two isozymes and contents of fatty acids, inorganic elements in Ge-enriched mycelia of Ganoderma lucidum, Chinese Pharmaceutical Journal 40 (2005) 383-385. [8] B.S. Mitrevski, J.T. Brenna, Y. Zhang, P.J. Marriott, Application of comprehensive two-dimensional gas chromatography to sterols analysis, J Chromatogr A 1214 (2008) 134-142. [9] Z. Moldovan, E. Jover, J.M. Bayona, Gas chromatographic and mass spectrometric methods for the characterisation of long-chain fatty acids: Application to wool wax extracts, Analytica Chimica Acta 465 (2002) 359-378. [10] K. Pedneault, P. Angers, A. Gosselin, R.J. Tweddell, Fatty acid profiles of polar and neutral lipids of ten species of higher basidiomycetes indigenous to eastern Canada, Mycological Research 112 (2008) 1428-1434. [11] J..J. Liu, W.H. Huang, M.L. Lv, J.P. Si, B.L. Guo, S.J. Li. Determination of Ergosterol in Ganoderma lucidum from Different Varieties and Cultured Tree Species by HPLC, Journal of Chinese Medicinal Materials 34 (2011) 187-190. [12] H.W. Seo, T.M. Hung, M. Na, H.J. Jung, J.C. Kim, J.S. Choi, J.H. Kim, H.K. Lee, I. Lee, K. Bae, M. Hattori, B.S. Min, Steroids and triterpenes from the fruit bodies of Ganoderma lucidum and their anti-complement activity, Archives of Pharmacal Research 32 (2009) 1573-1579. [13] N.C. Shantha, G.E. Napolitano, Gas chromatography of fatty acids, Journal of Chromatography 624 (1992) 37-51. [14] M. Shen, M. Xie, S. Nie, Y. Wan, J. Xie, Discrimination of different Ganoderma species and their region based on GC-MS profiles of sterols and pattern recognition techniques, Analytical Letters 44 (2011) 863-873.

3. 毕业设计（论文）进程安排

2016.1.4-2016.1.15 文献查阅，研究方案制定和开题报告写作 2016.1.16-2016.2.7 开题报告的修改与复核 2016.2.17-2016.3.1 实践内容的准备 2016.3.2-2016.5.10 实践 2016.5.11-2016.5.15 实践内容处理与结果分析 2016.5.16-2016.6.1 毕业论文书写