1. 毕业设计（论文）的内容和要求

1．文献查阅 掌握文献查阅的一般方法，学会使用计算机在中国期刊网，维普数据库，超星数字图书馆，elsevier、ncbi电子期刊，springer link全文电子期刊，pubmed等检索资源上查阅关于遗传转化体系构建的相关文献。

2．文献阅读及综述 阅读与课题相关的外文及中文文献，了解国内外的研究动态，撰写文献综述。

3．明确实验任务，拟定实验方案 根据所查阅文献的内容，确定实验内容及方案，拟定科学可行的研究计划。

2. 参考文献

[1] Lin L, Zhao B, Xie R, Lin W, Li H, Li Y, Shi M, Chen Y G, Springer T A, Chen X. Carbon nanotube-assisted optical activation of TGF-beta signaling by near-infrared light[J]. Nature Nanotechnology, 2015, 10: 465-471. [2] Goncalves M S. Fluorescent labeling of biomolecules with organic probes[J], Chemical reviews, 2009, 109: 190-212. [3] Arya H, Kaul Z, Wadhwa R. Quantum dots in bio-imaging: revolution by the small[J]. Bioehem biophys research communications, 2005, 329: 1173-1177. [4] Yang D Z, Chen Q F, Wang W X. Direct and indirect immunolabelling of HeLa cells with quantum dots[J]. Luminescence, 2008, 23: 169-174. [5] Xue X H, Pan J, Xie H M. Fluorescence detection of total count of eseheriehia coli and staphylococcus aureus on water-soluble CdSe quantum dots coupled with baterial[J]. Talanta, 2009, 77: 1808-1813. [6] Yong K T, Ding H, Roy I. Imaging pancreatic cancer using bioconjugated InP quantum dots[J]. ACS Nano, 2009, 3: 502-510. [7] Chang E, Thekkek N, Yu W W. Evaluation of quantum dot cytotoxicity based on intracellular uptake[J]. Small, 2006, 2: 1412-1417. [8] Tsay J M, Michalet X, New light on quantum dotcytotoxicity[J]. ACS Chemical biology, 2005, 12: 1159-1161. [9] Derfus A M, Chan W C W, Bhatia S N. Probing the cytotoxicity of semiconductor quantum dots[J]. Nano letters, 2004, 4:11-18. [10] Kim J, Park Y, Yoon T H. Photoxicity of CdSe/ZnSe quantum dots with surface coatings of 3-mercaptopropionic acid or tri-N-octylphosphine oxide/gum Arabic in Daphnia magna under environmentally relevant UV-B light[J]. Aquatic Toxicology, 2010, 97: 116-124. [11] Lin L S, Cong Z x, Cao J B, Ke K M, Peng Q L, Gao J, Yang H H, Liu G, Chen X. Multifunctional Fe3O4@polydopamine core-shell nanocompsites for intracellular Mrna detection and imaiging-guided photothermal therapy[J]. ACS Nano, 2014, 8: 3876-3883. [12] Liu J, Detrembleur C, De Pauw Giller M C, Mornet S, Jerome C, Duguet E. Gold nanorods coated with mesoporous silica shell as drug delivery system for remote near infrared light-activated release and potential phototherapy[J]. Small, 2015, 11: 2323-2332.

3. 毕业设计（论文）进程安排

2018.1.2-2018.1.19 文献查询和资料阅读 2018.1.19-2018.1.23 研究方案制定和开题报告写作 2018.1.24-2018.3.10 实践内容的准备 2018.3.11-2018.5.10 实践 2018.5.10-2018.5.15 实践内容处理与结果分析 2018.5.16-2018.6.1 毕业论文书写