1. 毕业设计（论文）的内容和要求

以生物基化学品5-羟甲基糠醛（5-hydroxymethylfurfural,HMF）作为底物，挖掘能够高效转化/降解HMF的微生物和酶催化剂，对于实现纤维素水解产物的生物脱毒和以HMF为原料生物催化法生产精细化学品具有很重要意义。

主要研究内容： 1）以HMF为底物对实验室已保存的微生物菌种进行筛选，发现能够利用HMF的微生物； 2）以HMF为唯一碳源从环境土壤样品中富集筛选能够降解HMF的微生物； 3）从HMF降解微生物中挖掘新型HMF氧化酶，对酶基因进行异源表达，对其催化特性进行研究。

2. 参考文献

1. N. Wierckx, F. Koopman, L. Bandounas, J. H. de Winde, H. J. Ruijssenaars, Isolation and characterization of Cupriavidus basilensis HMF14 for biological removal of inhibitors from lignocellulosic hydrolysate. Microbial Biotechnology. 3, 336#8211;343 (2010). 2. Y.-Z. Qin, M.-H. Zong, W.-Y. Lou, N. Li, Biocatalytic Upgrading of 5-Hydroxymethylfurfural (HMF) with Levulinic Acid to HMF Levulinate in Biomass-Derived Solvents. ACS Sustainable Chemistry Engineering. 4, 4050#8211;4054 (2016). 3. Y.-Z. Qin et al., Enzyme-catalyzed selective oxidation of 5-hydroxymethylfurfural (HMF) and separation of HMF and 2,5-diformylfuran using deep eutectic solvents. Green Chem. 17, 3718#8211;3722 (2015). 4. A. A. Rosatella et al., 5-Hydroxymethylfurfural (HMF) as a building block platform: Biological properties, synthesis and synthetic applications. Green Chemistry. 13, 754 (2011). 5. W. P. Dijkman, M. W. Fraaije, Discovery and characterization of a 5-hydroxymethylfurfural oxidase from Methylovorus sp. strain MP688. Applied and environmental microbiology. 80, 1082#8211;90 (2014). 6. M. P. J. van Deurzen, F. van Rantwijk, R. A. Sheldon, Chloroperoxidase-Catalyzed Oxidation of 5-Hydroxymethylfurfural. Journal of Carbohydrate Chemistry. 16, 299#8211;309 (1997). 7. S. M. McKenna, S. Leimk#252;hler, S. Herter, N. J. Turner, A. J. Carnell, Enzyme cascade reactions: synthesis of furandicarboxylic acid (FDCA) and carboxylic acids using oxidases in tandem. Green Chem. 17, 3271#8211;3275 (2015). 8. J. Carro et al., 5-hydroxymethylfurfural conversion by fungal aryl-alcohol oxidase and unspecific peroxygenase. The FEBS journal. 282, 3218#8211;29 (2015). 9. X.-Y. Zhang, M.-H. Zong, N. Li, Whole-cell biocatalytic selective oxidation of 5-hydroxymethylfurfural to 5-hydroxymethyl-2-furancarboxylic acid. Green Chem. 19, 4544#8211;4551 (2017).

3. 毕业设计（论文）进程安排

5、研究进度安排 2018.1.8#8212;2018.2.28 确定课题，对课题相关文献进行调研，文献查找，包括国内外研究现状，最新进展。

实验室规章制度培训，安全培训以及各种仪器的使用。

2018.3.1#8212;2018.4.30 1) 从土壤样品中分离筛选出可降解hmf的微生物，从实验室保藏的菌种中筛选可降解hmf的微生物； 2) 将微生物分离得到纯化菌株，并富集培养； 3) 观测每种菌株的生长特性，及降解hmf的能力，选育优势菌株。