1. 毕业设计（论文）的内容和要求

1．文献查阅 掌握文献查阅，使用网络资源如中国期刊网、维普数据库、超星数字图书馆、elsevier电子期刊、pub med等全文电子期刊等检索资源，查阅关木犀草素、雷公藤甲素和睾丸支持细胞损伤相关方面的相关文献。

2．文献阅读及综述 阅读与课题相关的外文与中文文献，了解国内外的研究动态，撰写文献综述。

3．明确实验任务，拟定实验方案 根据所查阅文献的内容，确定实验内容及方案，拟定科学可行的研究计划。

2. 参考文献

[1] Wan, H. T.; Mruk, D. D.; Wong, C. K.; Cheng, C. Y. Perfluorooctanesulfonate (PFOS) perturbs male rat Sertoli cell blood-testis barrier function by affecting F-actin organization via p-FAK-Tyr(407): an in vitro study. Endocrinology 155:249-262; 2014. [2] Cao, X. N.; Shen, L. J.; Wu, S. D.; Yan, C.; Zhou, Y.; Xiong, G.; Wang, Y. C.; Liu, Y.; Liu, B.; Tang, X. L.; Guo, M.; Liu, D. Y.; Long, C. L.; Sun, M.; He, D. W.; Lin, T.; Wei, G. H. Urban fine particulate matter exposure causes male reproductive injury through destroying blood-testis barrier (BTB) integrity. Toxicol Lett 266:1-12; 2017. [3] Aravindakshan, J.; Cyr, D. G. Nonylphenol alters connexin 43 levels and connexin 43 phosphorylation via an inhibition of the p38-mitogen-activated protein kinase pathway. Biol Reprod 72:1232-1240; 2005. [4] Carette, D.; Perrard, M. H.; Prisant, N.; Gilleron, J.; Pointis, G.; Segretain, D.; Durand, P. Hexavalent chromium at low concentration alters Sertoli cell barrier and connexin 43 gap junction but not claudin-11 and N-cadherin in the rat seminiferous tubule culture model. Toxicol Appl Pharmacol 268:27-36; 2013. [5] Zhou, D. R.; Zhou, Y. C.; Cui, G. H.; Guo, X.; Qin, J.; Gui, Y. T.; Cai, Z. M. Gossypol repressed the gap junctional intercellular communication between Sertoli cells by decreasing the expression of Connexin43. Toxicol In Vitro 22:1719-1725; 2008. [6] Li, N.; Mruk, D. D.; Chen, H.; Wong, C. K.; Lee, W. M.; Cheng, C. Y. Rescue of perfluorooctanesulfonate (PFOS)-mediated Sertoli cell injury by overexpression of gap junction protein connexin 43. Sci Rep 6:29667; 2016. [7] Yang, D.; Tan, X.; Lv, Z.; Liu, B.; Baiyun, R.; Lu, J.; Zhang, Z. Regulation of Sirt1/Nrf2/TNF-α signaling pathway by luteolin is critical to attenuate acute mercuric chloride exposure induced hepatotoxicity. Sci Rep 6:37157; 2016. [8] Xu, J.; Wang, H.; Ding, K.; Zhang, L.; Wang, C.; Li, T.; Wei, W.; Lu, X. Luteolin provides neuroprotection in models of traumatic brain injury via the Nrf2-ARE pathway. Free Radic Biol Med 71:186-195; 2014. [9] Hong, Z.; Cao, X.; Li, N.; Zhang, Y.; Lan, L.; Zhou, Y.; Pan, X.; Shen, L.; Yin, Z.; Luo, L. Luteolin is effective in the non-small cell lung cancer model with L858R/T790M EGF receptor mutation and erlotinib resistance. Br J Pharmacol 171:2842-2853; 2014. [10] Liu, C. W.; Lin, H. W.; Yang, D. J.; Chen, S. Y.; Tseng, J. K.; Chang, T. J.; Chang, Y. Y. Luteolin inhibits viral-induced inflammatory response in RAW264.7 cells via suppression of STAT1/3 dependent NF-κB and activation of HO-1. Free Radic Biol Med 95:180-189; 2016. [11] Bai, L.; Nong, Y.; Shi, Y.; Liu, M.; Yan, L.; Shang, J.; Huang, F.; Lin, Y.; Tang, H. Luteolin Inhibits Hepatitis B Virus Replication through Extracellular Signal-Regulated Kinase-Mediated Down-Regulation of Hepatocyte Nuclear Factor 4α Expression. Mol Pharm 13:568-577; 2016. [12] Hu, J.; Man, W.; Shen, M.; Zhang, M.; Lin, J.; Wang, T.; Duan, Y.; Li, C.; Zhang, R.; Gao, E.; Wang, H.; Sun, D. Luteolin alleviates post-infarction cardiac dysfunction by up-regulating autophagy through Mst1 inhibition. J Cell Mol Med 20:147-156; 2016. [13] Chu, Q.; Tian, X.; Lin, M.; Ye, J. Electromigration profiles of Cynomorium songaricum based on capillary electrophoresis with amperometric detection. J Agric Food Chem 54:7979-7983; 2006. [14] Qiu, L.; Zhang, X.; Zhang, X.; Zhang, Y.; Gu, J.; Chen, M.; Zhang, Z.; Wang, X.; Wang, S. L. Sertoli cell is a potential target for perfluorooctane sulfonate-induced reproductive dysfunction in male mice. Toxicol Sci 135:229-240; 2013. [15] Ma, B.; Qi, H.; Li, J.; Xu, H.; Chi, B.; Zhu, J.; Yu, L.; An, G.; Zhang, Q. Triptolide disrupts fatty acids and peroxisome proliferator-activated receptor (PPAR) levels in male mice testes followed by testicular injury: A GC-MS based metabolomics study. Toxicology 336:84-95; 2015. [16] Xu, L.; Qiu, Y.; Xu, H.; Ao, W.; Lam, W.; Yang, X. Acute and subacute toxicity studies on triptolide and triptolide-loaded polymeric micelles following intravenous administration in rodents. Food Chem Toxicol 57:371-379; 2013. [17] Ma, B.; Zhang, Q.; Wu, D.; Wang, Y. L.; Hu, Y. Y.; Cheng, Y. P.; Yang, Z. D.; Zheng, Y. Y.; Ying, H. J. Strontium fructose 1,6-diphosphate prevents bone loss in a rat model of postmenopausal osteoporosis via the OPG/RANKL/RANK pathway. Acta Pharmacol Sin 33:479-489; 2012. [18] Yang, Y.; Wang, W.; Xiong, Z.; Kong, J.; Qiu, Y.; Shen, F.; Huang, Z. Activation of SIRT3 attenuates triptolide-induced toxicity through closing mitochondrial permeability transition pore in cardiomyocytes. Toxicol In Vitro 34:128-137; 2016. [19] Hu, J.; Yu, Q.; Zhao, F.; Ji, J.; Jiang, Z.; Chen, X.; Gao, P.; Ren, Y.; Shao, S.; Zhang, L.; Yan, M. Protection of Quercetin against Triptolide-induced apoptosis by suppressing oxidative stress in rat Leydig cells. Chem Biol Interact 240:38-46; 2015. [20] Zhou, J.; Xi, C.; Wang, W.; Yang, Y.; Qiu, Y.; Huang, Z. Autophagy plays an important role in triptolide-induced apoptosis in cardiomyocytes. Toxicol Lett 236:168-183; 2015. [21] Zhou, J.; Xi, C.; Wang, W.; Fu, X.; Jinqiang, L.; Qiu, Y.; Jin, J.; Xu, J.; Huang, Z. Triptolide-induced oxidative stress involved with Nrf2 contribute to cardiomyocyte apoptosis through mitochondrial dependent pathways. Toxicol Lett 230:454-466; 2014. [22] Zhang, Y. C.; Gan, F. F.; Shelar, S. B.; Ng, K. Y.; Chew, E. H. Antioxidant and Nrf2 inducing activities of luteolin, a flavonoid constituent in Ixeris sonchifolia Hance, provide neuroprotective effects against ischemia-induced cellular injury. Food Chem Toxicol 59:272-280; 2013. [23] Kittiratphatthana, N.; Kukongviriyapan, V.; Prawan, A.; Senggunprai, L. Luteolin induces cholangiocarcinoma cell apoptosis through the mitochondrial-dependent pathway mediated by reactive oxygen species. J Pharm Pharmacol 68:1184-1192; 2016. [24] Stanton, P. G. Regulation of the blood-testis barrier. Semin Cell Dev Biol 59:166-173; 2016. [25] Gerber, J.; Weider, K.; Hambruch, N.; Brehm, R. Loss of connexin43 (Cx43) in Sertoli cells leads to spatio-temporal alterations in occludin expression. Histol Histopathol 29:935-948; 2014. [26] Li, M. W.; Mruk, D. D.; Lee, W. M.; Cheng, C. Y. Connexin 43 is critical to maintain the homeostasis of the blood-testis barrier via its effects on tight junction reassembly. Proc Natl Acad Sci U S A 107:17998-18003; 2010. [27] Kidder, G. M.; Cyr, D. G. Roles of connexins in testis development and spermatogenesis. Semin Cell Dev Biol 50:22-30; 2016. [28] Li, N.; Mruk, D. D.; Mok, K. W.; Li, M. W.; Wong, C. K.; Lee, W. M.; Han, D.; Silvestrini, B.; Cheng, C. Y. Connexin 43 reboots meiosis and reseals blood-testis barrier following toxicant-mediated aspermatogenesis and barrier disruption. FASEB J 30:1436-1452; 2016. [29] Chen, Z.; Xie, X.; Huang, J.; Gong, W.; Zhu, X.; Chen, Q.; Huang, J.; Huang, H. Connexin43 regulates high glucose-induced expression of fibronectin, ICAM-1 and TGF-β1 via Nrf2/ARE pathway in glomerular mesangial cells. Free Radic Biol Med 102:77-86; 2017.

3. 毕业设计（论文）进程安排

2月 确定毕业设计的课题及相关资料的查找 3月 完成相关的材料准备；完成开题报告 3月 实验方法的确定及实验条件的优化 4月 实验阶段 5月 各项指标作比较，实验数据处理 6月 毕业论文的书写 6月 在导师指导下修改论文，完成答辩